BMRB Entry 11581

Title:
NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion
Deposition date:
2014-11-06
Original release date:
2016-07-13
Authors:
Mahajan, Mukesh; Bhattacharjya, Surajit
Citation:

Citation: Mahajan, Mukesh; Bhattacharjya, Surajit. "NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion"  Biochim. Biophys. Acta ., .-..

Assembly members:

Assembly members:
Pre_transmembrane_domain, polymer, 18 residues, 2378.794 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pre_transmembrane_domain: LGKYEQYIKWPWYVWLGF

Data sets:
Data typeCount
13C chemical shifts16
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pre transmembrane domain1

Entities:

Entity 1, Pre transmembrane domain 18 residues - 2378.794 Da.

1   LEUGLYLYSTYRGLUGLNTYRILELYSTRP
2   PROTRPTYRVALTRPLEUGLYPHE

Samples:

sample_1: Pre transmembrane domain 0.5 mM; DPC, [U-99% 2H], 125 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 4.7; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz