BMRB Entry 11537

Title:
Solution structure of actinomycin
Deposition date:
2013-10-16
Original release date:
2014-10-20
Authors:
Umetsu, Yoshitaka; Ohki, Shinya
Citation:

Citation: Umetsu, Yoshitaka; Ohki, Shinya. "Solution structure of actinomycin"  .

Assembly members:

Assembly members:
entity, polymer, 36 residues, 4078.634 Da.

Natural source:

Natural source:   Common Name: Actinomyces sp.   Taxonomy ID: 29317   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Actinomyces sp.

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GFGCPWNAYECDRHCVSKGY TGGNCRGKIRQTCHCY

Data sets:
Data typeCount
1H chemical shifts189

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 36 residues - 4078.634 Da.

1   GLYPHEGLYCYSPROTRPASNALATYRGLU
2   CYSASPARGHISCYSVALSERLYSGLYTYR
3   THRGLYGLYASNCYSARGGLYLYSILEARG
4   GLNTHRCYSHISCYSTYR

Samples:

sample_1: entity 2 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 3.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB EFW28532
REF WP_009393172