BMRB Entry 11500

Title:
solution structure of PARP11 WWE domain
Deposition date:
2012-05-08
Original release date:
2018-12-18
Authors:
He, Fahu; Muto, Yutaka; Inoue, Makoto; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Yokoyama, Shigeyuki
Citation:

Citation: He, Fahu; Tsuda, Kengo; Takahashi, Mari; Kuwasako, Kanako; Terada, Takano; Shirouzu, Mikako; Watanabe, Satoru; Kigawa, Takanori; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the interaction of ADP-ribose with the PARP WWE domains."  FEBS Lett. 586, 3858-3864 (2012).
PubMed: 23010590

Assembly members:

Assembly members:
PARP11, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P-000001

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts100
1H chemical shifts641

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WWE1

Entities:

Entity 1, WWE 102 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNGLUVAL
2   ASPASPMETASPTHRSERASPTHRGLNTRP
3   GLYTRPPHETYRLEUALAGLUCYSGLYLYS
4   TRPHISMETPHEGLNPROASPTHRASNSER
5   GLNCYSSERVALSERSERGLUASPILEGLU
6   LYSSERPHELYSTHRASNPROCYSGLYSER
7   ILESERPHETHRTHRSERLYSPHESERTYR
8   LYSILEASPPHEALAGLUMETLYSGLNMET
9   ASNLEUTHRTHRGLYLYSGLNARGLEUILE
10   LYSARGALAPROPHESERSERGLYPROSER
11   SERGLY

Samples:

sample_1: PARP11, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayahsi - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks