BMRB Entry 11402

Title:
Solution structure of chromo domain 2 in Chromodomain-helicase-DNA-binding protein 6
Deposition date:
2010-09-09
Original release date:
2011-09-08
Authors:
Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation:

Citation: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of chromo domain 2 in Chromodomain-helicase-DNA-binding protein 6"  .

Assembly members:

Assembly members:
Chromo domain, UNP residues 371-431, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060904-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromo domain, UNP residues 371-431: GSSGSSGNPDYVEVDRILEV AHTKDAETGEEVTHYLVKWC SLPYEESTWELEEDVDPAKV KEFESLQV

Data sets:
Data typeCount
13C chemical shifts287
15N chemical shifts63
1H chemical shifts441

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chromo domain, UNP residues 371-4311

Entities:

Entity 1, Chromo domain, UNP residues 371-431 68 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNPROASP
2   TYRVALGLUVALASPARGILELEUGLUVAL
3   ALAHISTHRLYSASPALAGLUTHRGLYGLU
4   GLUVALTHRHISTYRLEUVALLYSTRPCYS
5   SERLEUPROTYRGLUGLUSERTHRTRPGLU
6   LEUGLUGLUASPVALASPPROALALYSVAL
7   LYSGLUPHEGLUSERLEUGLNVAL

Samples:

sample_1: Chromo domain, UNP residues 371-431, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9825, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG59500
GB AAI72397 AAK56405 AAN59903 EAW75974 EAW75975
REF NP_001230229 NP_115597 XP_001502820 XP_002721122 XP_002806815
SP Q8TD26
AlphaFold Q8TD26

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks