BMRB Entry 11388

Title:
Solution structure of the ARID domain of Jarid1b protein
Deposition date:
2010-09-08
Original release date:
2011-09-07
Authors:
Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation:

Citation: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the ARID domain of Jarid1b protein"  .

Assembly members:

Assembly members:
ARID domain, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060821-07

Data sets:
Data typeCount
13C chemical shifts522
15N chemical shifts118
1H chemical shifts835

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ARID domain1

Entities:

Entity 1, ARID domain 122 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUALAGLN
2   THRARGVALLYSLEUASNPHELEUASPGLN
3   ILEALALYSTYRTRPGLULEUGLNGLYSER
4   THRLEULYSILEPROHISVALGLUARGLYS
5   ILELEUASPLEUPHEGLNLEUASNLYSLEU
6   VALALAGLUGLUGLYGLYPHEALAVALVAL
7   CYSLYSASPARGLYSTRPTHRLYSILEALA
8   THRLYSMETGLYPHEALAPROGLYLYSALA
9   VALGLYSERHISILEARGGLYHISTYRGLU
10   ARGILELEUASNPROTYRASNLEUPHELEU
11   SERGLYASPSERLEUARGCYSLEUGLNLYS
12   PROASNLEUTHRSERASPTHRLYSASPLYS
13   GLUTYR

Samples:

sample_1: ARID domain, [U-13C; U-15N], 0.77 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 15732
PDB
DBJ BAC30898 BAD90482 BAE37363
EMBL CAB43532 CAB63108
GB AAD16061 AAH48180 AAH57318 AAI56050 AAI57032
REF NP_001100647 NP_001300971 NP_006609 NP_690855 XP_002717636
SP Q80Y84 Q9UGL1
TPG DAA21261
AlphaFold Q9UGL1 Q80Y84

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks