BMRB Entry 11339

Title:
Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens"  .

Assembly members:

Assembly members:
RING domain, polymer, 75 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060417-22

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts75
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, RING domain 75 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERLEUGLN
2   LYSGLUILESERTHRGLUGLUGLNLEUARG
3   ARGLEUGLNGLUGLULYSLEUCYSLYSILE
4   CYSMETASPARGASNILEALAILEVALPHE
5   VALPROCYSGLYHISLEUVALTHRCYSLYS
6   GLNCYSALAGLUALAVALASPLYSCYSPRO
7   METCYSTYRTHRVALILETHRPHELYSGLN
8   LYSILEPHEMETSER

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - refinement

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG36609 BAI46620
GB AAC50373 AAC50518 AAH32729 AAW62257 AAX29953
REF NP_001158 NP_001191330 XP_003262340 XP_003779771 XP_003823173
SP P98170
AlphaFold P98170

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks