BMRB Entry 11297

Title:
Solusion structure of the Todor domain of human Lamin-B receptor
Deposition date:
2010-08-09
Original release date:
2011-08-19
Authors:
Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solusion structure of the Todor domain of human Lamin-B receptor"  .

Assembly members:

Assembly members:
Tudor domain, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-15

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts59
1H chemical shifts408

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tudor domain1

Entities:

Entity 1, Tudor domain 68 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETPROSER
2   ARGLYSPHEALAASPGLYGLUVALVALARG
3   GLYARGTRPPROGLYSERSERLEUTYRTYR
4   GLUVALGLUILELEUSERHISASPSERTHR
5   SERGLNLEUTYRTHRVALLYSTYRLYSASP
6   GLYTHRGLULEUGLULEULYSGLUASNASP
7   ILELYSSERGLYPROSERSERGLY

Samples:

sample_1: Tudor domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9736, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAD92751 BAD96554 BAG10816 BAG35190 BAG64605
EMBL CAH92286
GB AAA59494 AAA59495 AAH20079 ABM81975 ABM85153
REF NP_001127533 NP_001248262 NP_002287 NP_919424 XP_002760653
SP Q14739 Q5R7H4
AlphaFold Q14739 Q5R7H4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks