BMRB Entry 11280

Title:
2DZJ/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Synaptic Glycoprotein SC2
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Zhao, C.; Yoneyama, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Yoneyama, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "2DZJ/Solution Structure of the N-terminal Ubiquitin-like Domain in Human Synaptic Glycoprotein SC2"  .

Assembly members:

Assembly members:
ubiquitin-like domain, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060522-14

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts393
15N chemical shifts82
1H chemical shifts602

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin-like domain1

Entities:

Entity 1, ubiquitin-like domain 88 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETLYSHIS
2   TYRGLUVALGLUILELEUASPALALYSTHR
3   ARGGLULYSLEUCYSPHELEUASPLYSVAL
4   GLUPROHISALATHRILEALAGLUILELYS
5   ASNLEUPHETHRLYSTHRHISPROGLNTRP
6   TYRPROALAARGGLNSERLEUARGLEUASP
7   PROLYSGLYLYSSERLEULYSASPGLUASP
8   VALLEUGLNLYSLEUPROVALGLYTHRTHR
9   ALATHRLEUTYRPHEARGASPLEU

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9748, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE88553 BAE90144 BAG37802 BAG50909 BAI47191
EMBL CAG33173
GB AAF32373 AAH00174 AAH02425 AAH05952 AAH07801
REF NP_001029920 NP_001231519 NP_001270331 NP_612510 XP_001498421
SP Q3ZCD7 Q9NZ01
TPG DAA28092
AlphaFold Q9NZ01 Q3ZCD7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks