BMRB Entry 11273

Title:
Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein"  .

Assembly members:

Assembly members:
UBA-like domain, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050815-24

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts211
15N chemical shifts54
1H chemical shifts334

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBA-like domain1

Entities:

Entity 1, UBA-like domain 62 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYSERALA
2   ALASERSERALALEULYSGLYLEUILEGLN
3   GLNPHETHRTHRILETHRGLYALASERGLU
4   SERVALGLYLYSHISMETLEUGLUALACYS
5   ASNASNASNLEUGLUMETALAVALTHRMET
6   PHELEUASPGLYGLYGLYSERGLYPROSER
7   SERGLY

Samples:

sample_1: UBA-like domain, [U-13C; U-15N], 0.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
GB EDM11425 EDM11426 EDM11427 KFM11413 KFO08321
REF NP_001100556 XP_003477156 XP_004003056 XP_004644633 XP_004712317

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks