BMRB Entry 11237

Title:
Solution structure of the first and second PHD domain from Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first and second PHD domain from Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog"  .

Assembly members:

Assembly members:
PHD domain, polymer, 111 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P070115-52

Data sets:
Data typeCount
13C chemical shifts454
15N chemical shifts113
1H chemical shifts699

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PHD domain1
2ZINC ION no.12
3ZINC ION no.22
4ZINC ION no.32
5ZINC ION no.42

Entities:

Entity 1, PHD domain 111 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAASNCYS
2   ALAVALCYSASPSERPROGLYASPLEULEU
3   ASPGLNPHEPHECYSTHRTHRCYSGLYGLN
4   HISTYRHISGLYMETCYSLEUASPILEALA
5   VALTHRPROLEULYSARGALAGLYTRPGLN
6   CYSPROGLUCYSLYSVALCYSGLNASNCYS
7   LYSGLNSERGLYGLUASPSERLYSMETLEU
8   VALCYSASPTHRCYSASPLYSGLYTYRHIS
9   THRPHECYSLEUGLNPROVALMETLYSSER
10   VALPROTHRASNGLYTRPLYSCYSLYSASN
11   CYSARGILECYSILESERGLYPROSERSER
12   GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PHD domain, [U-13C; U-15N], 0.94 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAC35712
REF XP_007948343 XP_010639685 XP_010639691 XP_010639698

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks