BMRB Entry 11228

Title:
Solution structure of the SH3 domain from Rho guanine nucleotide exchange factor 9
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain from Rho guanine nucleotide exchange factor 9"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050919-06

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts83
1H chemical shifts506

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 81 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPSERILE
2   VALSERALAGLUALAVALTRPASPHISVAL
3   THRMETALAASNARGGLULEUALAPHELYS
4   ALAGLYASPVALILELYSVALLEUASPALA
5   SERASNLYSASPTRPTRPTRPGLYGLNILE
6   ASPASPGLUGLUGLYTRPPHEPROALASER
7   PHEVALARGLEUTRPVALASNGLNGLUASP
8   GLUVALGLUGLUGLYSERGLYPROSERSER
9   GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 0.46 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA24854 BAC30147 BAC65562 BAE24002 BAE37525
EMBL CAH90157
GB AAI17407 AAI41386 AAX46427 ADR83209 AIC50931
REF NP_001028501 NP_001030495 NP_001125046 NP_001277313 NP_001277314
SP O43307 Q3UTH8 Q58DL7 Q5RDK0
TPG DAA12786
AlphaFold O43307 Q3UTH8 Q58DL7 Q5RDK0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks