BMRB Entry 11227

Name: Solution structure of the second fn3 domain from human Ephrin type-B receptor 4
Published: 2011-08-03

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PDB ID: 2e7h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11227
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the second fn3 domain from human Ephrin type-B receptor 4

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second fn3 domain from human Ephrin type-B receptor 4" .

Assembly members:

Assembly members:
FN3 domain, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050919-21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FN3 domain: GSSGSSGPPAVSDIRVTRSS PSSLSLAWAVPRAPSGAVLD YEVKYHEKGAEGPSSVRFLK TSENRAELRGLKRGASYLVQ VRARSEAGYGPFGQEHHSQT QLDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	428
15N chemical shifts	94
1H chemical shifts	665

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FN3 domain	1

Entities:

Entity 1, FN3 domain 109 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

ALA

2

VAL

SER

ASP

ILE

ARG

VAL

THR

ARG

SER

3

PRO

SER

LEU

SER

LEU

ALA

TRP

ALA

VAL

4

PRO

ARG

ALA

PRO

SER

GLY

ALA

VAL

LEU

ASP

5

TYR

GLU

VAL

LYS

TYR

HIS

GLU

LYS

GLY

ALA

6

GLU

GLY

PRO

SER

VAL

ARG

PHE

LEU

LYS

7

THR

SER

GLU

ASN

ARG

ALA

GLU

LEU

ARG

GLY

8

LEU

LYS

ARG

GLY

ALA

SER

TYR

LEU

VAL

GLN

9

VAL

ARG

ALA

ARG

SER

GLU

ALA

GLY

TYR

GLY

10

PRO

PHE

GLY

GLN

GLU

HIS

SER

GLN

THR

11

GLN

LEU

ASP

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 0.80 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2E7H
DBJ	BAD92881
GB	AAA20598 AAH04264 AAH52804 AAK21010 AAL14194
REF	NP_001193197 NP_004435 XP_002711965 XP_002803271 XP_002806627
SP	P54760
TPG	DAA15196
AlphaFold	P54760