BMRB Entry 11224

Name: Solution structure of the SH3 domain from Phospholipase C, gamma 2
Published: 2011-08-03

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PDB ID: 2eqi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11224
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain from Phospholipase C, gamma 2

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Nagashima, T.; Hayahsi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain from Phospholipase C, gamma 2" .

Assembly members:

Assembly members:
SH3 domain, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050815-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGRTVKALYDYKAKR SDELTFCRGALIHNVSKEPG GWWKGDYGTRIQQYFPSNYV EDISGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	284
15N chemical shifts	63
1H chemical shifts	430

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 69 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

THR

VAL

2

LYS

ALA

LEU

TYR

ASP

TYR

LYS

ALA

LYS

ARG

3

SER

ASP

GLU

LEU

THR

PHE

CYS

ARG

GLY

ALA

4

LEU

ILE

HIS

ASN

VAL

SER

LYS

GLU

PRO

GLY

5

GLY

TRP

LYS

GLY

ASP

TYR

GLY

THR

ARG

6

ILE

GLN

TYR

PHE

PRO

SER

ASN

TYR

VAL

7

GLU

ASP

ILE

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2EQI
DBJ	BAD92151 BAG10628 BAG65485
EMBL	CAA32194
GB	AAA41896 AAA60112 AAH07565 AAH11772 AAH14561
REF	NP_002652 NP_058864 NP_758489 XP_001148089 XP_001502048
SP	P16885 P24135 Q8CIH5
AlphaFold	P24135 P16885 Q8CIH5