BMRB Entry 11133

Title:
Solution structure of the fourth CH domain from human L-plastin
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the fourth CH domain from human L-plastin"  .

Assembly members:

Assembly members:
CH domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050111-01

Data sets:
Data typeCount
13C chemical shifts497
15N chemical shifts117
1H chemical shifts806

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CH domain1

Entities:

Entity 1, CH domain 124 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNASPASP
2   ILEILEVALASNTRPVALASNGLUTHRLEU
3   ARGGLUALAGLULYSSERSERSERILESER
4   SERPHELYSASPPROLYSILESERTHRSER
5   LEUPROVALLEUASPLEUILEASPALAILE
6   GLNPROGLYSERILEASNTYRASPLEULEU
7   LYSTHRGLUASNLEUASNASPASPGLULYS
8   LEUASNASNALALYSTYRALAILESERMET
9   ALAARGLYSILEGLYALAARGVALTYRALA
10   LEUPROGLUASPLEUVALGLUVALASNPRO
11   LYSMETVALMETTHRVALPHEALACYSLEU
12   METGLYLYSGLYMETLYSARGVALSERGLY
13   PROSERSERGLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAD97025 BAG35310
GB AAA63236 AAB02845 AAH07673 AAH10271 ABM83861
REF XP_001157685 XP_001158161 XP_002824296 XP_002824297 XP_003270105

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks