BMRB Entry 11063

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for KP-PmrD
Deposition date:
2008-12-30
Original release date:
2010-01-12
Authors:
Luo, Shih-Chi
Citation:

Citation: Luo, Shih-Chi; Chen, Chinpan. "Structural characterization of PmrD, which connects the PhoPQ and PmrAB two-component signal-transduction systems"  .

Assembly members:

Assembly members:
PmrD, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 484021   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET 29b(+)

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts75
1H chemical shifts510

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Polymyxin b resistant protein D1

Entities:

Entity 1, Polymyxin b resistant protein D 89 residues - Formula weight is not available

1   METGLUTRPTRPVALLYSLYSVALGLNASP
2   ASNALASERALASERLEUCYSARGVALVAL
3   LEUGLNSERGLYALALEUGLUMETILEALA
4   GLUILEGLUALACYSARGLEUARGLEUARG
5   GLUGLYASPLYSLEUTHRPROLEUALAASP
6   ALAARGTYRCYSLEUASNASNASNPROTHR
7   GLNTHRLEULYSILEARGASNALATHRHIS
8   TYRSERSERGLUARGTRPTHRASNALAASP
9   LYSLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Polymyxin b resistant protein D, [U-95% 13C; U-90% 15N], 0.4 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAH64168
EMBL CCI77623 CCM84637 CCM87409 CCM96230 CCN30508
GB ABR77864 AEJ98926 AEW62201 AFQ65083 AGT23550
REF WP_004141189 WP_004157936 WP_014907299 WP_015874957 WP_023284006

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks