BMRB Entry 11044

Title:
Solution structure of Magi4, a spider toxin from Macrothele gigas
Deposition date:
2008-04-13
Original release date:
2009-07-17
Authors:
Yamaji, Nahoko
Citation:

Citation: Yamaji, Nahoko; Little, Michelle; Nishio, Hideki; Billen, Bert; Villegas, Elba; Nishiuchi, Yuji; Tytgat, Jan; Nicholson, Graham; Corzo, Gerardo. "Synthesis, Solution Structure, and Phylum Selectivity of a Spider {delta}-Toxin That Slows Inactivation of Specific Voltage-gated Sodium Channel Subtypes"  J. Biol. Chem. 284, 24568-24582 (2009).
PubMed: 19592486

Assembly members:

Assembly members:
magi4, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Macrothele gigas   Taxonomy ID: 223896   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrothele gigas

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
magi4: CGSKRAWCKEKKDCCCGYNC VYAWYNQQSSCERKWKYLFT GEC

Data sets:
Data typeCount
1H chemical shifts286

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1magi41

Entities:

Entity 1, magi4 43 residues - Formula weight is not available

1   CYSGLYSERLYSARGALATRPCYSLYSGLU
2   LYSLYSASPCYSCYSCYSGLYTYRASNCYS
3   VALTYRALATRPTYRASNGLNGLNSERSER
4   CYSGLUARGLYSTRPLYSTYRLEUPHETHR
5   GLYGLUCYS

Samples:

sample_1: magi4 3.7 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 2.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.1, Schwieters, Kuszewski, Tjandra and Clore - structure solution

ANSIG v3.3, Kraulis - chemical shift assignment, peak picking

xwinnmr v2.5, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAC80149
SP P83560
AlphaFold P83560