BMRB Entry 10277

Title:
Solution structure of the Chromo domain of chromobox homolog 2 from human
Deposition date:
2008-12-15
Original release date:
2009-12-14
Authors:
Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Chromo domain of chromobox homolog 2 from human"  .

Assembly members:

Assembly members:
Chromo domain, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050613-29

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts70
1H chemical shifts492

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chromo domain1

Entities:

Entity 1, Chromo domain 74 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUGLNVAL
2   PHEALAALAGLUCYSILELEUSERLYSARG
3   LEUARGLYSGLYLYSLEUGLUTYRLEUVAL
4   LYSTRPARGGLYTRPSERSERLYSHISASN
5   SERTRPGLUPROGLUGLUASNILELEUASP
6   PROARGLEULEULEUALAPHEGLNLYSLYS
7   GLUHISGLULYSGLUVALGLNASNSERGLY
8   PROSERSERGLY

Samples:

sample_1: chromo domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE34242 BAG73012 BAK20222
EMBL CAA44398 CAA73723
GB AAH04252 AAH35199 AAI19760 AAI19761 AAI56119
REF NP_001100541 NP_001192522 NP_005180 NP_031649 NP_116036
SP P30658 Q14781
TPG DAA18115
AlphaFold P30658 Q14781

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks