BMRB Entry 10255

Name: Solution structure of the N-terminal PH domain of ARAP2 protein from human
Published: 2009-11-20

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PDB ID: 2cod
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10255
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal PH domain of ARAP2 protein from human

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal PH domain of ARAP2 protein from human" .

Assembly members:

Assembly members:
PH domain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050125-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGKVKSGWLDKLSPQ GKRMFQKRWVKFDGLSISYY NNEKEMYSKGIIPLSAISTV RVQGDNKFEVVTTQRTFVFR VEKEEERNDWISILLNALKS QSLTSQSQASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	465
15N chemical shifts	101
1H chemical shifts	731

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 115 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LYS

VAL

LYS

2

SER

GLY

TRP

LEU

ASP

LYS

LEU

SER

PRO

GLN

3

GLY

LYS

ARG

MET

PHE

GLN

LYS

ARG

TRP

VAL

4

LYS

PHE

ASP

GLY

LEU

SER

ILE

SER

TYR

5

ASN

GLU

LYS

GLU

MET

TYR

SER

LYS

GLY

6

ILE

PRO

LEU

SER

ALA

ILE

SER

THR

VAL

7

ARG

VAL

GLN

GLY

ASP

ASN

LYS

PHE

GLU

VAL

8

VAL

THR

GLN

ARG

THR

PHE

VAL

PHE

ARG

9

VAL

GLU

LYS

GLU

ARG

ASN

ASP

TRP

10

ILE

SER

ILE

LEU

ASN

ALA

LEU

LYS

SER

11

GLN

SER

LEU

THR

SER

GLN

SER

GLN

ALA

SER

12

GLY

PRO

SER

GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 0.62 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2COD
DBJ	BAA25506 BAG10357
GB	AAI50259 AAI60097 AAL12170 EAW92870 EAW92872
REF	NP_056045 XP_001090397 XP_001135477 XP_003832197 XP_003898614
SP	Q8WZ64
AlphaFold	Q8WZ64