BMRB Entry 10087

Title:
Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)
Deposition date:
2007-01-24
Original release date:
2008-08-14
Authors:
Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)"  .

Assembly members:

Assembly members:
ubiquitin like domain, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040301-13

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts348
15N chemical shifts87
1H chemical shifts550

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
159 kDa 2'-5'-oligoadenylate synthetase like protein1

Entities:

Entity 1, 59 kDa 2'-5'-oligoadenylate synthetase like protein 87 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILEGLNVAL
2   PHEVALLYSASNPROASPGLYGLYSERTYR
3   ALATYRALAILEASNPROASNSERPHEILE
4   LEUGLYLEULYSGLNGLNILEGLUASPGLN
5   GLNGLYLEUPROLYSLYSGLNGLNGLNLEU
6   GLUPHEGLNGLYGLNVALLEUGLNASPTRP
7   LEUGLYLEUGLYILETYRGLYILEGLNASP
8   SERASPTHRLEUILELEUSERLYSLYSLYS
9   GLYSERGLYPROSERSERGLY

Samples:

sample_1: ubiquitin like domain, [U-13C; U-15N], 1.1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8994, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAK62869
REF NP_001267398 XP_003907322 XP_004054078 XP_004054079

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks