BMRB Entry 10086

Title:
RA domain of guanine nucleotide exchange factor for Rap1
Deposition date:
2007-01-24
Original release date:
2008-08-14
Authors:
Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "RA domain of guanine nucleotide exchange factor for Rap1"  .

Assembly members:

Assembly members:
RA domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030804-93

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts86
1H chemical shifts684

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rap guanine nucleotide exchange factor 51

Entities:

Entity 1, Rap guanine nucleotide exchange factor 5 104 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUGLUILE
2   PHECYSHISVALTYRILETHRGLUHISSER
3   TYRVALSERVALLYSALALYSVALSERSER
4   ILEALAGLNGLUILELEULYSVALVALALA
5   GLULYSILEGLNTYRALAGLUGLUASPLEU
6   ALALEUVALALAILETHRPHESERGLYGLU
7   LYSHISGLULEUGLNPROASNASPLEUVAL
8   ILESERLYSSERLEUGLUALASERGLYARG
9   ILETYRVALTYRARGLYSASPLEUALAASP
10   THRLEUASNPROPHEALAGLUASNSERGLY
11   PROSERSERGLY

Samples:

sample_1: RA domain, [U-13C; U-15N], 1.04 mM; d-TRIS 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA13406 BAG09689 BAG58663
GB EAW93745 EAW93746
REF XP_011513954 XP_012303210
SP Q92565
AlphaFold Q92565

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks