BMRB Entry 10085

Title:
Solution structure of the first RRM domain in heterogeneous nuclear ribonucleoprotein H
Deposition date:
2007-01-24
Original release date:
2008-08-14
Authors:
He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation:

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of the first RRM domain in heterogeneous nuclear ribonucleoprotein H"  .

Assembly members:

Assembly members:
RRM domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: p040223-24

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts96
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Heterogeneous nuclear ribonucleoprotein H1

Entities:

Entity 1, Heterogeneous nuclear ribonucleoprotein H 104 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNSERPRO
2   ASPTHRALAASNASPGLYPHEVALARGLEU
3   ARGGLYLEUPROPHEGLYCYSSERLYSGLU
4   GLUILEVALGLNPHEPHESERGLYLEUGLU
5   ILEVALPROASNGLYMETTHRLEUPROVAL
6   ASPPHEGLNGLYARGSERTHRGLYGLUALA
7   PHEVALGLNPHEALASERGLNGLUILEALA
8   GLULYSALALEULYSLYSHISLYSGLUARG
9   ILEGLYHISARGTYRILEGLUILEPHELYS
10   SERSERARGALAGLUVALARGTHRSERGLY
11   PROSERSERGLY

Samples:

sample_1: RRM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.29, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks