BMRB Entry 10064

Title:
Solution Structure of The forth PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of The forth PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)"  .

Assembly members:

Assembly members:
PDZ Domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P021030-32

Data sets:
Data typeCount
13C chemical shifts467
15N chemical shifts109
1H chemical shifts759

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2)1

Entities:

Entity 1, MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2) 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERLEUGLN
2   THRSERASPVALVALILEHISARGLYSGLU
3   ASNGLUGLYPHEGLYPHEVALILEILESER
4   SERLEUASNARGPROGLUSERGLYSERTHR
5   ILETHRVALPROHISLYSILEGLYARGILE
6   ILEASPGLYSERPROALAASPARGCYSALA
7   LYSLEULYSVALGLYASPARGILELEUALA
8   VALASNGLYGLNSERILEILEASNMETPRO
9   HISALAASPILEVALLYSLEUILELYSASP
10   ALAGLYLEUSERVALTHRLEUARGILEILE
11   PROGLNGLUGLULEUASNSERPROSERGLY
12   PROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.4 mM; Tris-HCl(7.0) 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.820, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks