BMRB Entry 10044

Title:
Solution structure of homeobox domain of Arabidopsisthaliana zinc finger homeobox family protein
Deposition date:
2006-11-09
Original release date:
2008-08-13
Authors:
Kaneno, D.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Kaneno, D.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of homeobox domain of Arabidopsisthaliana zinc finger homeobox family protein"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030107-56

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts78
1H chemical shifts498

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZF-HD homeobox family protein1

Entities:

Entity 1, ZF-HD homeobox family protein 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERSERALA
2   GLUALAGLYGLYGLYILEARGLYSARGHIS
3   ARGTHRLYSPHETHRALAGLUGLNLYSGLU
4   ARGMETLEUALALEUALAGLUARGILEGLY
5   TRPARGILEGLNARGGLNASPASPGLUVAL
6   ILEGLNARGPHECYSGLNGLUTHRGLYVAL
7   PROARGGLNVALLEULYSVALTRPLEUHIS
8   ASNASNLYSHISSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-15N, U-13C], 1.1 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsample_1not availablecondition_1
3D_15N-separated_NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.811, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB11563
GB AAO00745 AAP13412 AED98048
REF NP_201344
SP Q9FKP8
AlphaFold Q9FKP8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks