BMRB Entry 10013

Title:
Solution Structure Of The Ring Finger Domain Of The Human Kiaa1045 Protein
Deposition date:
2005-11-20
Original release date:
2006-12-06
Authors:
Miyamoto, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING Finger Domain of the Human KIAA1045 Protein"  not known ., .-..

Assembly members:

Assembly members:
RING Finger Domain, polymer, 89 residues, 9688.757 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030609-29

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts340
15N chemical shifts76
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING Finger Domain1
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, RING Finger Domain 89 residues - 9688.757 Da.

1   GLYSERSERGLYSERSERGLYPROARGGLU
2   PROVALVALASNASPGLUMETCYSASPVAL
3   CYSGLUVALTRPTHRALAGLUSERLEUPHE
4   PROCYSARGVALCYSTHRARGVALPHEHIS
5   ASPGLYCYSLEUARGARGMETGLYTYRILE
6   GLNGLYASPSERALAALAGLUVALTHRGLU
7   METALAHISTHRGLUTHRGLYTRPSERCYS
8   HISTYRCYSASPASNILEASNLEULEULEU
9   THRGLUGLUSERGLYPROSERSERGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING Finger Domain, [U-13C; U-15N], 0.4 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; D2O 10%; H2O 90%

condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsample_1not availablecondition_1
3D_15N-separated_NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.8996 - data analysis

CYANA v2.0.17 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA82997 BAE90222 BAE90573 BAG09947 BAH11739
EMBL CAH93249 CAI29683
GB EAW58408 EHH24010 EHH57286 ELR52775
REF NP_001127105 NP_001128900 NP_001291262 NP_056112 XP_002689719
SP Q5R4R7 Q9UPV7
TPG DAA26823
AlphaFold Q9UPV7 Q5R4R7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks