BMRB Entry 7264

Title:
Backbone and Selected Sidechain 1H, 13C, and 15N Chemical Shift Assignments of the beta-1,4-glycosidase Cex from Cellulomonas fimi
Deposition date:
2006-08-15
Original release date:
2006-11-13
Authors:
Poon, David; Ludwiczek, Martin; Schubert, Mario; Withers, Stephen; McIntosh, Lawrence
Citation:

Citation: Poon, David; Ludwiczek, Martin; Schubert, Mario; Kwan, Emily; Withers, Stephen; McIntosh, Lawrence. "NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate."  Biochemistry 46, 1759-1770 (2007).
PubMed: 17253772

Assembly members:

Assembly members:
apo-CexCD, polymer, 315 residues, 34230 Da.

Natural source:

Natural source:   Common Name: Cellulomonas fimi   Taxonomy ID: 1708   Superkingdom: Bacteria   Kingdom: Actinobacteria   Genus/species: Cellulomonas fimi

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pUC12-1.1Cex(PTIS)

Data sets:
Data typeCount
13C chemical shifts939
15N chemical shifts301
1H chemical shifts306

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cex catalytic domain1

Entities:

Entity 1, Cex catalytic domain 315 residues - 34230 Da.

1   ALATHRTHRLEULYSGLUALAALAASPGLY
2   ALAGLYARGASPPHEGLYPHEALALEUASP
3   PROASNARGLEUSERGLUALAGLNTYRLYS
4   ALAILEALAASPSERGLUPHEASNLEUVAL
5   VALALAGLUASNALAMETLYSTRPASPALA
6   THRGLUPROSERGLNASNSERPHESERPHE
7   GLYALAGLYASPARGVALALASERTYRALA
8   ALAASPTHRGLYLYSGLULEUTYRGLYHIS
9   THRLEUVALTRPHISSERGLNLEUPROASP
10   TRPALALYSASNLEUASNGLYSERALAPHE
11   GLUSERALAMETVALASNHISVALTHRLYS
12   VALALAASPHISPHEGLUGLYLYSVALALA
13   SERTRPASPVALVALASNGLUALAPHEALA
14   ASPGLYGLYGLYARGARGGLNASPSERALA
15   PHEGLNGLNLYSLEUGLYASNGLYTYRILE
16   GLUTHRALAPHEARGALAALAARGALAALA
17   ASPPROTHRALALYSLEUCYSILEASNASP
18   TYRASNVALGLUGLYILEASNALALYSSER
19   ASNSERLEUTYRASPLEUVALLYSASPPHE
20   LYSALAARGGLYVALPROLEUASPCYSVAL
21   GLYPHEGLNSERHISLEUILEVALGLYGLN
22   VALPROGLYASPPHEARGGLNASNLEUGLN
23   ARGPHEALAASPLEUGLYVALASPVALARG
24   ILETHRGLULEUASPILEARGMETARGTHR
25   PROSERASPALATHRLYSLEUALATHRGLN
26   ALAALAASPTYRLYSLYSVALVALGLNALA
27   CYSMETGLNVALTHRARGCYSGLNGLYVAL
28   THRVALTRPGLYILETHRASPLYSTYRSER
29   TRPVALPROASPVALPHEPROGLYGLUGLY
30   ALAALALEUVALTRPASPALASERTYRALA
31   LYSLYSPROALATYRALAALAVALMETGLU
32   ALAPHEGLYALASER

Samples:

apo_CexCD: apo-CexCD, [70% 2H; U-99% 13C; U-99% 15N], 0.4 mM; potassium phosphate 20 mM; sodium azide 0.02%

NMR_buffering_condition: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSY HSQCapo_CexCDnot availableNMR_buffering_condition
HNCAapo_CexCDnot availableNMR_buffering_condition
HN(CO)CAapo_CexCDnot availableNMR_buffering_condition
HNCOapo_CexCDnot availableNMR_buffering_condition
HN(CA)COapo_CexCDnot availableNMR_buffering_condition
HN(CA)CBapo_CexCDnot availableNMR_buffering_condition
HN(COCA)CBapo_CexCDnot availableNMR_buffering_condition
NN-NOESYapo_CexCDnot availableNMR_buffering_condition
HN-NOESYapo_CexCDnot availableNMR_buffering_condition

Software:

NMRPipe -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 7265
PDB
GB AAA56791 AAA56792 AEA30147 AEE45406
PRF 1211268A
REF WP_013770432 YP_004452793
SP P07986
AlphaFold P07986

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks