BMRB Entry 6940

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6940

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of the 2H, 13C, 15N labeled amino-terminal domain of apo-pantothenate synthetase from E.coli.

Deposition date:

2006-01-16

Original release date:

2006-06-26

Authors:

Chakrabarti, Kalyan Sundar; Sarma, Siddhartha

Citation:

Citation: Chakrabarti, Kalyan Sundar; Sarma, Siddhartha. "NMR Assignment of 2H, 13C and 15N Labeled Amino-Terminal Domain of Apo-Pantothenate Synthetase from E. coli" J. Biomol. NMR 36, 38-38 (2006).
PubMed: 16705359

Assembly members:

Assembly members:
panc, polymer, 176 residues, 19830 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
panc: MLIIETLPLLRQQIRRLRME GKRVALVPTMGNLHDGHMKL VDEAKARADVVAVSIFVNPM QFDRPEDLARYPRTLQEDCE KLNKRKVDLVFAPSVKEIYP NGTETHTYVDVPGLSTMLEG ASRPGHFRGVSTIVSKLFNL VQPDIACFGEKDFQQLALIR KMVADMGFDIEIVGVP

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	470
15N chemical shifts	152
1H chemical shifts	152

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	amino-terminal pantothenate synthetase, chain 1	1
2	amino-terminal pantothenate synthetase, chain 2	1

Entities:

Entity 1, amino-terminal pantothenate synthetase, chain 1 176 residues - 19830 Da.

1

MET

LEU

ILE

GLU

THR

LEU

PRO

LEU

2

ARG

GLN

ILE

ARG

LEU

ARG

MET

GLU

3

GLY

LYS

ARG

VAL

ALA

LEU

VAL

PRO

THR

MET

4

GLY

ASN

LEU

HIS

ASP

GLY

HIS

MET

LYS

LEU

5

VAL

ASP

GLU

ALA

LYS

ALA

ARG

ALA

ASP

VAL

6

VAL

ALA

VAL

SER

ILE

PHE

VAL

ASN

PRO

MET

7

GLN

PHE

ASP

ARG

PRO

GLU

ASP

LEU

ALA

ARG

8

TYR

PRO

ARG

THR

LEU

GLN

GLU

ASP

CYS

GLU

9

LYS

LEU

ASN

LYS

ARG

LYS

VAL

ASP

LEU

VAL

10

PHE

ALA

PRO

SER

VAL

LYS

GLU

ILE

TYR

PRO

11

ASN

GLY

THR

GLU

THR

HIS

THR

TYR

VAL

ASP

12

VAL

PRO

GLY

LEU

SER

THR

MET

LEU

GLU

GLY

13

ALA

SER

ARG

PRO

GLY

HIS

PHE

ARG

GLY

VAL

14

SER

THR

ILE

VAL

SER

LYS

LEU

PHE

ASN

LEU

15

VAL

GLN

PRO

ASP

ILE

ALA

CYS

PHE

GLY

GLU

16

LYS

ASP

PHE

GLN

LEU

ALA

LEU

ILE

ARG

17

LYS

MET

VAL

ALA

ASP

MET

GLY

PHE

ASP

ILE

18

GLU

ILE

VAL

GLY

VAL

PRO

Samples:

sample_1: panc, [U-90% 2H; U->98% 13C; U->98% 15N], 0.6 ± 0.05 mM; K2HPO4/KH2PO4 buffer 10 mM; NaCl 10 mM; DTT (Dithiothreitol) 1 mM

conditions_1: ionic strength: 10 mM; pH: 6.8; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
TROSY_1H15N_HSQC	sample_1	not available	conditions_1
TROSY_HNCA	sample_1	not available	conditions_1
TROSY_HN(CO)CA	sample_1	not available	conditions_1
TROSY_HNCACB	sample_1	not available	conditions_1
TROSY_HN(CO)CACB	sample_1	not available	conditions_1
TROSY-HNCO	sample_1	not available	conditions_1
3D_NOE_HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 700 MHz

PDB	1IHO 3GUZ
DBJ	BAB33560 BAE76042 BAG75657 BAI23496 BAI29011
EMBL	CAP74704 CAQ30648 CAQ87737 CAQ97020 CAR01508
GB	AAA24272 AAC73244 AAG54437 AAN41793 AAN78658
REF	NP_285829 NP_308164 NP_414675 NP_706086 NP_752114
SP	A1A7H9 A7ZHM3 A7ZW82 B1IQK7 B1LGT5
AlphaFold	A7ZW82 A1A7H9 A7ZHM3 B1IQK7 B1LGT5