BMRB Entry 6806

Title:
Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex
Deposition date:
2005-09-01
Original release date:
2006-03-10
Authors:
Dogan, Jakob; Lendel, Christofer; Hard, Torleif
Citation:

Citation: Dogan, Jakob; Lendel, Christofer; Hard, Torleif. "NMR Assignments of the Free and Bound-state Protein Components of an Anti-idiotypic Affibody Complex"  J. Biomol. NMR 36, 13-13 (2006).
PubMed: 16456703

Assembly members:

Assembly members:
Ztaq, polymer, 58 residues, 6484.2 Da.
anti-Ztaq, polymer, 58 residues, 6419.3 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts134
1H chemical shifts855

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ztaq1
2anti-Ztaq2

Entities:

Entity 1, Ztaq 58 residues - 6484.2 Da.

1   VALASPASNLYSPHEASNLYSGLULEUGLY
2   TRPALATHRTRPGLUILEPHEASNLEUPRO
3   ASNLEUASNGLYVALGLNVALLYSALAPHE
4   ILEASPSERLEUARGASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Entity 2, anti-Ztaq 58 residues - 6419.3 Da.

1   VALASPASNLYSPHEASNLYSGLUARGVAL
2   ILEALAILEGLYGLUILEMETARGLEUPRO
3   ASNLEUASNSERLEUGLNVALVALALAPHE
4   ILEASNSERLEUARGASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Samples:

sample_1: Ztaq 2.0 mM; anti-Ztaq, [U-13C; U-15N], 1.5 mM; Potassium Phosphate 20 mM; Sodium Chloride 50 mM; Sodium Azide 0.01%; D2O 10 % v/v

sample_2: Ztaq, [U-13C; U-15N], 1.3 mM; anti-Ztaq 1.6 mM; Potassium Phosphate 20 mM; Sodium Chloride 50 mM; Sodium Azide 0.01%; D2O 10 % v/v

conditions_1: pH*: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
(H)CC(CO)NHnot availablenot availablenot available
15N-NOESY HSQCnot availablenot availablenot available
15N-TOCSY HSQCnot availablenot availablenot available
13C-NOESY HSQCnot availablenot availablenot available

Software:

ANSIG for WINDOWS - assignment, Data analysis

XWIN-NMR - processing

NMRPipe - processing

NMR spectrometers:

  • BRUKER AVANCE 600 MHz
  • BRUKER AVANCE 500 MHz
  • VARIAN INOVA 800 MHz

Related Database Links:

BMRB 6805
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks