BMRB Entry 6748

Title:
NMR assignments of the Ki67FHA/hNIFK(226-269)3P complex
Deposition date:
2005-07-25
Original release date:
2007-01-29
Authors:
Byeon, In-Ja; Gronenborn, Angela; Li, Hongyuan; Tsai, Ming-Daw
Citation:

Citation: Byeon, In-Ja; Li, Hongyuan; Song, Haiyan; Gronenborn, Angela; Tsai, Ming-Daw. "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67."  Nat. Struct. Mol. Biol. 12, 987-993 (2005).

Assembly members:

Assembly members:
Ki67FHA, polymer, 120 residues, Formula weight is not available
hNIFK, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts520
15N chemical shifts168
1H chemical shifts1055

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ki67FHA1
2hNIFK2

Entities:

Entity 1, Ki67FHA 120 residues - Formula weight is not available

1   METTRPPROTHRARGARGLEUVALTHRILE
2   LYSARGSERGLYVALASPGLYPROHISPHE
3   PROLEUSERLEUSERTHRCYSLEUPHEGLY
4   ARGGLYILEGLUCYSASPILEARGILEGLN
5   LEUPROVALVALSERLYSGLNHISCYSLYS
6   ILEGLUILEHISGLUGLNGLUALAILELEU
7   HISASNPHESERSERTHRASNPROTHRGLN
8   VALASNGLYSERVALILEASPGLUPROVAL
9   ARGLEULYSHISGLYASPVALILETHRILE
10   ILEASPARGSERPHEARGTYRGLUASNGLU
11   SERLEUGLNASNGLYARGLYSSERTHRGLU
12   PHEPROARGLYSILEARGGLUGLNGLUPRO

Entity 2, hNIFK 120 residues - Formula weight is not available

1   LYSTHRVALASPSERGLNGLYPROTHRPRO
2   VALCYSTHRPROTHRPHELEUGLUARGARG
3   LYSSERGLNVALALAGLULEUASNASPASP
4   ASPLYSASPASPGLUILEVALPHELYSGLN
5   PROILESERCYS

Samples:

sample_1: Ki67FHA, [U-13C; U-15N], 1 mM; hNIFK 1 mM

sample_2: Ki67FHA 1 mM; hNIFK, [U-13C; U-15N], 1 mM

conditions_1: pH: 7.4; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    DBJ BAB41210 BAF84592 BAI47080
    GB AAH12457 AAH22990 AAH24238 AAY14830 ABM82604
    REF NP_115766 XP_003818352 XP_004031768 XP_009242042 XP_515769
    SP Q9BYG3
    AlphaFold Q9BYG3

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks