BMRB Entry 6711

Title:
NMR assignment of Human Ubiquitin Conjugating Enzyme (Ubc7)
Deposition date:
2005-06-30
Original release date:
2005-10-26
Authors:
Briggman, Kathryn; Majumdar, Ananya; Coleman, Catherine; Chau, Vincent; Tolman, Joel
Citation:

Citation: Briggman, Kathryn; Majumdar, Ananya; Coleman, Catherine; Chau, Vincent; Tolman, Joel. "NMR Assignment of Human Ubiquitin Conjugating Enzyme Ubc7"  J. Biomol. NMR 32, 340-340 (2005).
PubMed: 16211496

Assembly members:

Assembly members:
hUbc7, polymer, 165 residues, 18504 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts645
15N chemical shifts147
1H chemical shifts923

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubc71

Entities:

Entity 1, Ubc7 165 residues - 18504 Da.

1   METALAGLYTHRALALEULYSARGLEUMET
2   ALAGLUTYRLYSGLNLEUTHRLEUASNPRO
3   PROGLUGLYILEVALALAGLYPROMETASN
4   GLUGLUASNPHEPHEGLUTRPGLUALALEU
5   ILEMETGLYPROGLUASPTHRCYSPHEGLU
6   PHEGLYVALPHEPROALAILELEUSERPHE
7   PROLEUASPTYRPROLEUSERPROPROLYS
8   METARGPHETHRCYSGLUMETPHEHISPRO
9   ASNILETYRPROASPGLYARGVALCYSILE
10   SERILELEUHISALAPROGLYASPASPPRO
11   METGLYTYRGLUSERSERALAGLUARGTRP
12   SERPROVALGLNSERVALGLULYSILELEU
13   LEUSERVALVALSERMETLEUALAGLUPRO
14   ASNASPGLUSERGLYALAASNVALASPALA
15   SERLYSMETTRPARGASPASPARGGLUGLN
16   PHETYRLYSILEALALYSGLNILEVALGLN
17   LYSSERLEUGLYLEU

Samples:

sample_1: hUbc7, [U-13C; U-15N], 1 mM; Tris buffer 50 mM; TCEP 2 mM; NaN3 0.05%; D2O 10%

sample_2: hUbc7, [U-2H; U-13C; U-15N], 1 mM; Tris buffer 10 mM; TCEP 2 mM; NaN3 0.05%; D2O 10%

conditions_1: ionic strength: 50 mM; pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HMQC-NOESY-HSQCsample_1not availableconditions_1
HBHA(CO)NHsample_2not availableconditions_1
H(CCCO)NH-TOCSYsample_1not availableconditions_1
CC(CO)NH-TOCSYsample_2not availableconditions_1
(H)CC(CO)NH-TOCSYsample_1not availableconditions_1
15N-edited_NOESY-HSQCsample_1not availableconditions_1

Software:

NMRView, One Moon Scientific, Inc. - assignment analysis

NMRPipe, NIH - data processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks