BMRB Entry 6700

Name: Resonance Assignments of 30 kDa complexes of TFIID subunit TAF1 with TATA-binding protein
Published: 2005-10-28

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6700

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance Assignments of 30 kDa complexes of TFIID subunit TAF1 with TATA-binding protein

Deposition date:

2005-06-22

Original release date:

2005-10-28

Authors:

Mal, Tapas; Liu, Dingjiang; Masutomi, Yutaka; Zheng, Le; Nakatani, Yoshihiro; Kokubo, Tetsuro; Ikura, Mitsuhiko

Citation:

Citation: Mal, Tapas; Liu, Dingjiang; Masutomi, Yutaka; Zheng, Le; Nakatani, Yoshihiro; Kokubo, Tetsuro; Ikura, Mitsuhiko. "Resonance Assignments of 30 kDa Complexes of TFIID Subunit TAF1 with TATA-binding Protein" J. Biomol. NMR 33, 76-76 (2005).
PubMed: 16222567

Assembly members:

Assembly members:
dTAF1, polymer, 67 residues, Formula weight is not available
TBPc, polymer, 192 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Drosophila Melanogaster Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dTAF1: EGSIGNGLDLTGILFGNIDS EGRLLQDDDGEGRGGTGFDA ELRENIGSLSKLGLDSMLLE VIDLKEA
TBPc: AAPESEKDTSATSGIVPTLQ NIVATVTLGCRLDLKTVALH ARNAEYNPKRFAAVIMRIRE PKTTALIFASGKMVVTGAKS EDDSKLASRKYARIIQKIGF AAKFTDFKIQNIVGSCDVKF PIRLEGLAFSHGTFSSYEPE LFPGLIYRMVKPKIVLLIFV SGKIVLTGAKQREEIYQAFE AIYPVLSEFRKM

Data sets:

assigned_chemical_shifts
- Chemical_shift_assignment_of_dTAF1(11-77)

Data type	Count
13C chemical shifts	600
15N chemical shifts	250
1H chemical shifts	601

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TBP associated factor 1 (TAF1)	1
2	yeast TATA binding protein (y)TBP	2

Entities:

Entity 1, TBP associated factor 1 (TAF1) 67 residues - Formula weight is not available

1

GLU

GLY

SER

ILE

GLY

ASN

GLY

LEU

ASP

LEU

2

THR

GLY

ILE

LEU

PHE

GLY

ASN

ILE

ASP

SER

3

GLU

GLY

ARG

LEU

GLN

ASP

GLY

4

GLU

GLY

ARG

GLY

THR

GLY

PHE

ASP

ALA

5

GLU

LEU

ARG

GLU

ASN

ILE

GLY

SER

LEU

SER

6

LYS

LEU

GLY

LEU

ASP

SER

MET

LEU

GLU

7

VAL

ILE

ASP

LEU

LYS

GLU

ALA

Entity 2, yeast TATA binding protein (y)TBP 192 residues - Formula weight is not available

1

ALA

PRO

GLU

SER

GLU

LYS

ASP

THR

SER

2

ALA

THR

SER

GLY

ILE

VAL

PRO

THR

LEU

GLN

3

ASN

ILE

VAL

ALA

THR

VAL

THR

LEU

GLY

CYS

4

ARG

LEU

ASP

LEU

LYS

THR

VAL

ALA

LEU

HIS

5

ALA

ARG

ASN

ALA

GLU

TYR

ASN

PRO

LYS

ARG

6

PHE

ALA

VAL

ILE

MET

ARG

ILE

ARG

GLU

7

PRO

LYS

THR

ALA

LEU

ILE

PHE

ALA

SER

8

GLY

LYS

MET

VAL

THR

GLY

ALA

LYS

SER

9

GLU

ASP

SER

LYS

LEU

ALA

SER

ARG

LYS

10

TYR

ALA

ARG

ILE

GLN

LYS

ILE

GLY

PHE

11

ALA

LYS

PHE

THR

ASP

PHE

LYS

ILE

GLN

12

ASN

ILE

VAL

GLY

SER

CYS

ASP

VAL

LYS

PHE

13

PRO

ILE

ARG

LEU

GLU

GLY

LEU

ALA

PHE

SER

14

HIS

GLY

THR

PHE

SER

TYR

GLU

PRO

GLU

15

LEU

PHE

PRO

GLY

LEU

ILE

TYR

ARG

MET

VAL

16

LYS

PRO

LYS

ILE

VAL

LEU

ILE

PHE

VAL

17

SER

GLY

LYS

ILE

VAL

LEU

THR

GLY

ALA

LYS

18

GLN

ARG

GLU

ILE

TYR

GLN

ALA

PHE

GLU

19

ALA

ILE

TYR

PRO

VAL

LEU

SER

GLU

PHE

ARG

20

LYS

MET

Samples:

dTAF1(11-77): dTAF1, [U-15N; U-13C; U-2H], 0.8 – 1 mM; TBPc, [U-15N; U-13C; U-2H], 0.8 – 1 mM; phosphate 20 mM; Glycerol 5 % (v/v); Na2SO4 250 mM; MgCl2 5 mM; DTT 10 mM; NaN3 0.05 mM

20_mM_phosphate_buffer: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC	dTAF1(11-77)	not available	20_mM_phosphate_buffer
HNCACA	dTAF1(11-77)	not available	20_mM_phosphate_buffer
CBCACONNH	dTAF1(11-77)	not available	20_mM_phosphate_buffer
HCCTOCSYNNH	dTAF1(11-77)	not available	20_mM_phosphate_buffer

Software:

No software information available

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

BMRB	6702
PDB	1NGM 1NH2 1RM1 1TBA 1TBP 1YTB 1YTF 4B0A
DBJ	GAA22978
EMBL	CAA34751 CAG60477 CAY79336 CCD25155 CCE61293
GB	AAA34458 AAA35146 AAA35147 AAB64675 AEP68354
PRF	1515352A
REF	NP_011075 XP_001646300 XP_003670398 XP_003683727 XP_003955666
SP	P13393
TPG	DAA07809
AlphaFold	P13393