BMRB Entry 6379

Title:
Backbone HN, N, C', CA, CB assignments of Escherichia coli SdiA1-171
Deposition date:
2004-11-08
Original release date:
2005-07-26
Authors:
Yao, Yong; Martinez-Yamout, Maria; Dyson, H.
Citation:

Citation: Yao, Yong; Martinez-Yamout, Maria; Dyson, H.. "Letter to the Editor: Backbone and side chain 1H, 13C and 15N assignments for Escherichia coli SdiA1-171, the autoinducer-binding domain of a quorum sensing protein"  J. Biomol. NMR 31, 373-374 (2005).
PubMed: 15929010

Assembly members:

Assembly members:
sdia, polymer, 171 residues, Formula weight is not available
N-(2-OXOTETRAHYDROFURAN-3-YL)OCTANAMIDE, non-polymer, 227.300 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts468
1H chemical shifts155
15N chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1quorum sensing protein1
2HSL_22

Entities:

Entity 1, quorum sensing protein 171 residues - Formula weight is not available

1   METSERASPLYSASPPHEPHESERTRPARG
2   ARGTHRMETLEULEUARGPHEGLNARGMET
3   GLUTHRALAGLUGLUVALTYRHISGLUILE
4   GLULEUGLNALAGLNGLNLEUGLUTYRASP
5   TYRTYRSERLEUCYSVALARGHISPROVAL
6   PROPHETHRARGPROLYSVALALAPHETYR
7   THRASNTYRPROGLUALATRPVALSERTYR
8   TYRGLNALALYSASNPHELEUALAILEASP
9   PROVALLEUASNPROGLUASNPHESERGLN
10   GLYHISLEUMETTRPASNASPASPLEUPHE
11   SERGLUALAGLNPROLEUTRPGLUALAALA
12   ARGALAHISGLYLEUARGARGGLYVALTHR
13   GLNTYRLEUMETLEUPROGLUARGALALEU
14   GLYPHELEUSERPHESERARGCYSSERALA
15   ARGGLUILEPROILELEUSERASPGLULEU
16   GLNLEULYSMETGLNLEULEUVALARGGLU
17   SERLEUMETALALEUMETARGLEUASNASP
18   GLU

Entity 2, HSL_2 - C12 H21 N O3 - 227.300 Da.

1   HTF

Samples:

sample_1: sdia, [U-13C; U-15N; U-2H], 0.3 mM

conditions_1: pH: 4.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CA)CBsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 6454
PDB
DBJ BAA15736 BAB36077 BAG77671 BAI26047 BAI30964
EMBL CAQ98852 CAR08247 CAR13401 CAU98011 CBG34902
GB AAC74983 AAG56931 ABV06323 ACA77373 ACB03106
REF NP_288377 NP_310681 NP_416426 WP_001152713 WP_001152714
SP P07026
AlphaFold P07026

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks