BMRB Entry 6369

Title:
1H, 13C 15N chemical shift assignment Bacillus halodurans Protein BH1534: The Northeast Structural Genomics Consortium Target BhR29
Deposition date:
2004-10-27
Original release date:
2008-08-28
Authors:
Liu, Gaohua; Ma, Lichung; Shen, Yang; Acton, Thomas; Atreya, Hanudatta; Xiao, Rong; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination"  Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).
PubMed: 16027363

Assembly members:

Assembly members:
Protein BH1534, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus halodurans   Taxonomy ID: 86665   Superkingdom: Bacteria   Kingdom: Not applicable   Genus/species: Bacillus halodurans

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts988
13C chemical shifts501
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein BH15341

Entities:

Entity 1, Protein BH1534 138 residues - Formula weight is not available

1   METTHRARGLEUPROASPILELYSLYSGLU
2   VALARGPHEASNALAPROILEGLULYSVAL
3   TRPGLUALAVALSERTHRSERGLUGLYLEU
4   ALAPHETRPPHEMETGLUASNASPLEULYS
5   ALAGLUTHRGLYHISHISPHEHISLEUGLN
6   SERPROPHEGLYPROSERPROCYSGLNVAL
7   THRASPVALGLUARGPROILELYSLEUSER
8   PHETHRTRPASPTHRASPGLYTRPSERVAL
9   THRPHEHISLEULYSGLUGLUGLUASNGLY
10   THRILEPHETHRILEVALHISSERGLYTRP
11   LYSGLNGLYASPTHRLYSVALGLULYSALA
12   GLYALAGLUSERALAVALVALHISGLUARG
13   METASPARGGLYTRPHISASPLEUVALASN
14   GLUARGLEUARGGLNILEVALGLU

Samples:

sample_1: Protein BH1534, [U-13C; U-15N], 0.8 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCabCanot availablenot availablenot available
GFT (4,3)D CabCacONHNnot availablenot availablenot available
GFT (4,3)D HabCabcoNHNnot availablenot availablenot available
GFT (4,3)HCCH and simutanously NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAB05253
REF NP_242400 WP_010897699

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks