BMRB Entry 6277

Title:
NMR solution structure of the ubiquitin conjugating enzyme UbcH5B
Deposition date:
2004-07-28
Original release date:
2005-02-21
Authors:
Houben, Klaartje; Dominguez, Cyril; van Schaik, Frederik; Timmers, Marc; Bonvin, Alexandre; Boelens, Rolf
Citation:

Citation: Houben, Klaartje; Dominguez, Cyril; van Schaik, Frederik; Timmers, Marc; Bonvin, Alexandre; Boelens, Rolf. "Solution structure of the ubiquitin-conjugating enzyme UbcH5B"  J. Mol. Biol. 344, 513-526 (2004).
PubMed: 15522302

Assembly members:

Assembly members:
UbcH5B, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Data sets:
Data typeCount
13C chemical shifts519
15N chemical shifts144
1H chemical shifts900

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH5B1

Entities:

Entity 1, UbcH5B 147 residues - Formula weight is not available

1   METALALEULYSARGILEHISLYSGLULEU
2   ASNASPLEUALAARGASPPROPROALAGLN
3   CYSSERALAGLYPROVALGLYASPASPMET
4   PHEHISTRPGLNALATHRILEMETGLYPRO
5   ASNASPSERPROTYRGLNGLYGLYVALPHE
6   PHELEUTHRILEHISPHEPROTHRASPTYR
7   PROPHELYSPROPROLYSVALALAPHETHR
8   THRARGILETYRHISPROASNILEASNSER
9   ASNGLYSERILECYSLEUASPILELEUARG
10   SERGLNTRPSERPROALALEUTHRILESER
11   LYSVALLEULEUSERILECYSSERLEULEU
12   CYSASPPROASNPROASPASPPROLEUVAL
13   PROGLUILEALAARGILETYRLYSTHRASP
14   ARGGLULYSTYRASNARGILEALAARGGLU
15   TRPTHRGLNLYSTYRALAMET

Samples:

sample: UbcH5B, [U-15N; U-13C], 0.3 mM; KCl 150 mM; Kpi 20 mM; ZnCl2 0.01 mM

sample_conditions: ionic strength: 0.15 M; pH: 7; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsamplenot availablesample_conditions
HNCACBsamplenot availablesample_conditions
HcCH-TOSCYsamplenot availablesample_conditions
hCCH-TOCSYsamplenot availablesample_conditions
1H15N_TOCSYsamplenot availablesample_conditions
1H15N_NOESYsamplenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4866
PDB
DBJ BAA87330 BAB23116 BAC28070 BAC33981 BAC36940
EMBL CAG31534 CAH93209 CAJ81618 CDQ70075 CDQ72611
GB AAA85100 AAA85101 AAA85102 AAA91460 AAA91461
PRF 2111484A
REF NP_001016064 NP_001026324 NP_001032369 NP_001039961 NP_001072141
SP P61077 P61078 P61079 P62837 P62838
TPG DAA27344
AlphaFold P61078 P61077 P61079 P62837 P62838

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks