BMRB Entry 6275

Title:
1H, 13C and 15N resonance assignments for the six-zinc finger domain of the MTF-1 metalloregulatory protein in the free state
Deposition date:
2004-07-26
Original release date:
2005-07-25
Authors:
Matskevich, Viktor; Potter, Belinda; Laity, John; Andrews, Glen; Knudsen, Nathan; Feng, Linda
Citation:

Citation: Potter, Belinda; Knudsen, Nathan; Feng, Linda; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "NMR assignment of the six zinc fingers of MTF-1 in the free and DNA bound states"  J. Biomol. NMR 31, 94-94 (2005).

Assembly members:

Assembly members:
metal-response element-binding transcription factor-1, polymer, 177 residues, 20367 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1040
13C chemical shifts748
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTF-1 monomer1
2ZINC (II) ION 12
3ZINC (II) ION 22
4ZINC (II) ION 32
5ZINC (II) ION 42
6ZINC (II) ION 52
7ZINC (II) ION 62

Entities:

Entity 1, MTF-1 monomer 177 residues - 20367 Da.

1   VALLYSARGTYRGLNCYSTHRPHEGLUGLY
2   CYSPROARGTHRTYRSERTHRALAGLYASN
3   LEUARGTHRHISGLNLYSTHRHISARGGLY
4   GLUTYRTHRPHEVALCYSASNGLNGLUGLY
5   CYSGLYLYSALAPHELEUTHRSERTYRSER
6   LEUARGILEHISVALARGVALHISTHRLYS
7   GLULYSPROPHEGLUCYSASPVALGLNGLY
8   CYSGLULYSALAPHEASNTHRLEUTYRARG
9   LEULYSALAHISGLNARGLEUHISTHRGLY
10   LYSTHRPHEASNCYSGLUSERGLNGLYCYS
11   SERLYSTYRPHETHRTHRLEUSERASPLEU
12   ARGLYSHISILEARGTHRHISTHRGLYGLU
13   LYSPROPHEARGCYSASPHISASPGLYCYS
14   GLYLYSALAPHEALAALASERHISHISLEU
15   LYSTHRHISVALARGTHRHISTHRGLYGLU
16   ARGPROPHEPHECYSPROSERASNGLYCYS
17   GLULYSTHRPHESERTHRGLNTYRSERLEU
18   LYSSERHISMETLYSGLYHIS

Entity 2, ZINC (II) ION 1 - Zn - 65.409 Da.

1   ZN

Samples:

all_samples: metal-response element-binding transcription factor-1, [U-98% 13C; U-98% 15N], 1.0 mM; 2-Morpholinoethanesulfonic acid 20 mM; H2O 95%; D2O 5%

Cond_1: pH: 6.9; temperature: 303 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
2D EXPERIMENTS:all_samplesnot availableCond_1
1H-15N HSQCall_samplesnot availableCond_1
3D EXPERIMENTS:all_samplesnot availableCond_1
HNCACBall_samplesnot availableCond_1
HNCAall_samplesnot availableCond_1
CBCA(CO)NHall_samplesnot availableCond_1
(HCA)CO(CA)NHall_samplesnot availableCond_1
HNCOall_samplesnot availableCond_1
3J-HNHAall_samplesnot availableCond_1
HCCH-TOCSYall_samplesnot availableCond_1
HCCH-COSYall_samplesnot availableCond_1
1H-15N-HSQC-NOESYall_samplesnot availableCond_1
1H-13C-HSQC-NOESY (two data sets with carbon-transmitter centered at 119 and 37 ppm)all_samplesnot availableCond_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 - Raw spectral data processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 6276 6445

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks