BMRB Entry 5858

Title:
Characterization of us-ms Dynamics of Proteins Using a Combined Analysis of 15N NMR Relaxation and Chemical Shift: Conformational Exchange in Plastocyanin Induced by Histidine Protonations
Deposition date:
2003-07-04
Original release date:
2004-02-12
Authors:
Hass, Mathias; Thuesen, Marianne; Christensen, Hans; Led, Jens
Citation:

Citation: Hass, Mathias; Thuesen, Marianne; Christensen, Hans; Led, Jens. "Characterization of us-ms Dynamics of Proteins Using a Combined Analysis of 15N NMR Relaxation and Chemical Shift: Conformational Exchange in Plastocyanin Induced by Histidine Protonations "  J. Am. Chem. Soc. 126, 753-765 (2004).
PubMed: 14733549

Assembly members:

Assembly members:
Cu(II) Plastocyanin from Anabeana Variabilis, polymer, 106 residues, 11900 Da.
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Anabaena variabilis   Taxonomy ID: 1172   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21G(DE3)

Entity Sequences (FASTA):

Data typeCount
1H chemical shifts1312
15N chemical shifts1312
T1 relaxation values742
T2 relaxation values570

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin monomer1
2COPPER (I) ION2

Entities:

Entity 1, plastocyanin monomer 106 residues - 11900 Da.

1   METGLUTHRTYRTHRVALLYSLEUGLYSER
2   ASPLYSGLYLEULEUVALPHEGLUPROALA
3   LYSLEUTHRILELYSPROGLYASPTHRVAL
4   GLUPHELEUASNASNLYSVALPROPROHIS
5   ASNVALVALPHEASPALAALALEUASNPRO
6   ALALYSSERALAASPLEUALALYSSERLEU
7   SERHISLYSGLNLEULEUMETSERPROGLY
8   GLNSERTHRSERTHRTHRPHEPROALAASP
9   ALAPROALAGLYGLUTYRTHRPHETYRCYS
10   GLUPROHISARGGLYALAGLYMETVALGLY
11   LYSILETHRVALALAGLY

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Cu(II) Plastocyanin from Anabeana Variabilis, [U-95% 15N], 1.0 mM; natrium ascorbate 0.1 mM

Condition_1: pH: 8.50 na; temperature: 298 K

Condition_2: pH: 7.80 na; temperature: 298 K

Condition_3: pH: 7.46 na; temperature: 298 K

Condition_4: pH: 6.96 na; temperature: 298 K

Condition_5: pH: 6.59 na; temperature: 298 K

Condition_6: pH: 6.05 na; temperature: 298 K

Condition_7: pH: 5.78 na; temperature: 298 K

Condition_8: pH: 5.35 na; temperature: 298 K

Condition_9: pH: 4.98 na; temperature: 298 K

Condition_10: pH: 4.60 na; temperature: 298 K

Condition_11: pH: 4.20 na; temperature: 298 K

Condition_12: pH: 5.78 na; temperature: 293 K

Condition_13: pH: 5.78 na; temperature: 303 K

Condition_14: pH: 5.78 na; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 800 MHz

Related Database Links:

SWISS-PROT P46444 P0C178 O52830
REF NP_484302
PIR CUAI
GenBank AAA59364
EMBL CAA05338
DBJ BAB77782
PDB
BMRB 5505 4738

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks