BMRB Entry 5856

Title:
Backbone Resonances Assignments of Human Adult Hemoglobin in the Carbonmonoxy Form
Deposition date:
2003-07-03
Original release date:
2004-02-11
Authors:
Lukin, Jonathan; Kontaxis, Georg; Simplaceanu, Virgil; Yuan, Yue; Bax, Ad; Ho, Chien
Citation:

Citation: Lukin, Jonathan; Kontaxis, Georg; Simplaceanu, Virgil; Yuan, Yue; Bax, Ad; Ho, Chien. "Letter to the Editor: Backbone Resonance Assignments of Human Adult Hemoglobin in the Carbonmonoxy Form"  J. Biomol. NMR 28, 203-204 (2004).
PubMed: 14755170

Assembly members:

Assembly members:
globin subunit alpha, polymer, 141 residues, Formula weight is not available
globin subunit beta, polymer, 146 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
CMO, non-polymer, 28.010 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHE2

Data sets:
Data typeCount
13C chemical shifts749
15N chemical shifts263
1H chemical shifts263

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1globin subunit alpha, one1
2globin subunit alpha, two1
3globin subunit beta, one2
4globin subunit beta, two2
5heme unit, one3
6heme unit, two3
7carbon monoxide, one4
8carbon monoxide, two4

Entities:

Entity 1, globin subunit alpha, one 141 residues - Formula weight is not available

1   VALLEUSERPROALAASPLYSTHRASNVAL
2   LYSALAALATRPGLYLYSVALGLYALAHIS
3   ALAGLYGLUTYRGLYALAGLUALALEUGLU
4   ARGMETPHELEUSERPHEPROTHRTHRLYS
5   THRTYRPHEPROHISPHEASPLEUSERHIS
6   GLYSERALAGLNVALLYSGLYHISGLYLYS
7   LYSVALALAASPALALEUTHRASNALAVAL
8   ALAHISVALASPASPMETPROASNALALEU
9   SERALALEUSERASPLEUHISALAHISLYS
10   LEUARGVALASPPROVALASNPHELYSLEU
11   LEUSERHISCYSLEULEUVALTHRLEUALA
12   ALAHISLEUPROALAGLUPHETHRPROALA
13   VALHISALASERLEUASPLYSPHELEUALA
14   SERVALSERTHRVALLEUTHRSERLYSTYR
15   ARG

Entity 2, globin subunit beta, one 146 residues - Formula weight is not available

1   VALHISLEUTHRPROGLUGLULYSSERALA
2   VALTHRALALEUTRPGLYLYSVALASNVAL
3   ASPGLUVALGLYGLYGLUALALEUGLYARG
4   LEULEUVALVALTYRPROTRPTHRGLNARG
5   PHEPHEGLUSERPHEGLYASPLEUSERTHR
6   PROASPALAVALMETGLYASNPROLYSVAL
7   LYSALAHISGLYLYSLYSVALLEUGLYALA
8   PHESERASPGLYLEUALAHISLEUASPASN
9   LEULYSGLYTHRPHEALATHRLEUSERGLU
10   LEUHISCYSASPLYSLEUHISVALASPPRO
11   GLUASNPHEARGLEULEUGLYASNVALLEU
12   VALCYSVALLEUALAHISHISPHEGLYLYS
13   GLUPHETHRPROPROVALGLNALAALATYR
14   GLNLYSVALVALALAGLYVALALAASNALA
15   LEUALAHISLYSTYRHIS

Entity 3, heme unit, one - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 4, carbon monoxide, one - C O - 28.010 Da.

1   CMO

Samples:

sample_one: globin subunit alpha, [U-2H; U-13C; U-15N], 1.0 mM; globin subunit beta, [U-2H; U-13C; U-15N], 1.0 mM; Na phosphate 0.1 M; H2O 90%; D2O 10%

sample_conditions: pH: 7.0; temperature: 302.0 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 1053 1102 2006 2708 2710 6230 6683 7125 908
PDB
DBJ BAG34767
EMBL CAA23756 CAA23758 CAA23759 CAA43421 CAG38767
GB AAA16334 AAA21100 AAA21101 AAA21102 AAA21103
PRF 0404170B 0907233B
REF NP_000509 XP_002822173 XP_003819077 XP_004050595 XP_004090697
SP P02024 P02025 P02032 P68871 P68872
AlphaFold P68871 P02024 P02025 P02032 P68872

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks