BMRB Entry 5760

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5760

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Title:
1H, 13C, and 15N backbone assignment of the first two Ig domains Z1Z2 of the giant muscle protein titin
Deposition date:
2003-04-01
Original release date:
2003-09-05
Authors:
Edlich, Christian; Muhle-Goll, Claudia
Citation:

Citation: Edlich, Christian; Muhle-Goll, Claudia. "Letter to the Editor: 1H, 13C, and 15N backbone assignment of the first two Ig domains Z1Z2 of the giant muscle protein titin"  J. Biomol. NMR 27, 283-284 (2003).

Assembly members:

Assembly members:
titin Z1Z2, polymer, 204 residues, 21924 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
titin Z1Z2: MKHHHHHHPMATQAPTFTQP LQSVVVLEGSTATFEAHISG FPVPEVSWFRDGQVISTSTL PGVQISFSDGRAKLTIPAVT KANSGRYSLKATNGSGQATS TAELLVKAETAPPNFVQRLQ SMTVRQGSQVRLQVRVTGIP TPVVKFYRDGAEIQSSLDFQ ISQEGDLYSLLIAEAYPEDS GTYSVNATNSVGRATSTAEL LVQG

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Z1Z21

Entities:

Entity 1, Z1Z2 204 residues - 21924 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   ALATHRGLNALAPROTHRPHETHRGLNPRO
3   LEUGLNSERVALVALVALLEUGLUGLYSER
4   THRALATHRPHEGLUALAHISILESERGLY
5   PHEPROVALPROGLUVALSERTRPPHEARG
6   ASPGLYGLNVALILESERTHRSERTHRLEU
7   PROGLYVALGLNILESERPHESERASPGLY
8   ARGALALYSLEUTHRILEPROALAVALTHR
9   LYSALAASNSERGLYARGTYRSERLEULYS
10   ALATHRASNGLYSERGLYGLNALATHRSER
11   THRALAGLULEULEUVALLYSALAGLUTHR
12   ALAPROPROASNPHEVALGLNARGLEUGLN
13   SERMETTHRVALARGGLNGLYSERGLNVAL
14   ARGLEUGLNVALARGVALTHRGLYILEPRO
15   THRPROVALVALLYSPHETYRARGASPGLY
16   ALAGLUILEGLNSERSERLEUASPPHEGLN
17   ILESERGLNGLUGLYASPLEUTYRSERLEU
18   LEUILEALAGLUALATYRPROGLUASPSER
19   GLYTHRTYRSERVALASNALATHRASNSER
20   VALGLYARGALATHRSERTHRALAGLULEU
21   LEUVALGLNGLY

Samples:

sample_1: titin Z1Z2, [U-100% 15N; U-100% 13C; U-70% 2H], 1.0 mM; sodium acetate 10 mM; (NH4)2SO4 50 mM

Ex-cond_1: ionic strength: 0.1 M; pH: 5.7; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks