BMRB Entry 5723

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Brazzein
Deposition date:
2003-03-10
Original release date:
2003-09-15
Authors:
Assadi-Porter, Fariba; Abilgaard, Frits; Blad, Heike; Markley, John
Citation:

Citation: Assadi-Porter, Fariba; Abilgaard, Frits; Blad, Heike; Markley, John. "Correlation of the Sweetness of Variants of the Protein Brazzein with Patterns of Hydrogen Bonds Detected by NMR Spectroscopy"  J. Biol. Chem. 278, 31331-31339 (2003).
PubMed: 12732626

Assembly members:

Assembly members:
brazzein, polymer, 53 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts50
15N chemical shifts50
1H chemical shifts50
coupling constants17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wt brazzein1

Entities:

Entity 1, wt brazzein 53 residues - Formula weight is not available

1   ASPLYSCYSLYSLYSVALTYRGLUASNTYR
2   PROVALSERLYSCYSGLNLEUALAASNGLN
3   CYSASNTYRASPCYSLYSLEUASPLYSHIS
4   ALAARGSERGLYGLUCYSPHETYRASPGLU
5   LYSARGASNLEUGLNCYSILECYSASPTYR
6   CYSGLUTYR

Samples:

sample_1: brazzein, [U-100% 13C; U-100% 15N], 2.0 – 4.0 mM; 2,2-dimethyl-2-silapentane-5-sulfonate 0.1 mM; sodium azide 1 mM

sample_2: brazzein, [U-100% 15N], 2.0 mM

condition_1: ionic strength: 2 mM; pH: 5.2 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCAnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H-15N TOCSYnot availablenot availablenot available
1H-1H-15N NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 16215 16978 18710 4067 5295 5724 5725 5726 5727 5728
PDB
GB ACK76425 AGM38242
SP P56552
AlphaFold P56552

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks