BMRB Entry 5700

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5700

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Title:
Backbone 1H, 13C and 15N resonance assignments for the 25.8 kDa DNA binding domain of the human p63 protein
Deposition date:
2003-02-18
Original release date:
2003-03-21
Authors:
Furrer, Julien; Enthart, Andreas; Dehner, Alexander; Klein, Christian; Hansen, Silke; Schwaiger, Manfred; Gemmecker, Gerd; Kessler, Horst
Citation:

Citation: Furrer, Julien; Enthart, Andreas; Kuhlewein, Angelika; Dehner, Alexander; Klein, Christian; Hansen, Silke; Schwaiger, Manfred; Gemmecker, Gerd; Kessler, Horst. "Backbone 1H, 13C and 15N resonance assignments for the 25.8 kDa DNA binding domain of the human p63 protein"  J. Biomol. NMR 26, 377-378 (2003).
PubMed: 12815266

Assembly members:

Assembly members:
p63, polymer, 233 residues, 25832 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p63: GSSTFDALSPSPAIPSNTDY PGPHSFDVSFQQSSTAKSAT WTYSTELKKLYCQIAKTCPI QIKVMTPPPQGAVIRAMPVY KKAEHVTEVVKRCPNHELSR EFNEGQIAPPSHLIRVEGNS HAQYVEDPITGRQSVLVPYE PPQVGTEFTTVLYNFMCNSS CVGGMNRRPILIIVTLETRD GQVLGRRCFEARICACPGRD RKADEDSIRKQQVSDSTKNG DAFRQNTHGIQMT

Data sets:
Data typeCount
13C chemical shifts608
15N chemical shifts200
1H chemical shifts370

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p63 DBD1

Entities:

Entity 1, p63 DBD 233 residues - 25832 Da.

1   GLYSERSERTHRPHEASPALALEUSERPRO
2   SERPROALAILEPROSERASNTHRASPTYR
3   PROGLYPROHISSERPHEASPVALSERPHE
4   GLNGLNSERSERTHRALALYSSERALATHR
5   TRPTHRTYRSERTHRGLULEULYSLYSLEU
6   TYRCYSGLNILEALALYSTHRCYSPROILE
7   GLNILELYSVALMETTHRPROPROPROGLN
8   GLYALAVALILEARGALAMETPROVALTYR
9   LYSLYSALAGLUHISVALTHRGLUVALVAL
10   LYSARGCYSPROASNHISGLULEUSERARG
11   GLUPHEASNGLUGLYGLNILEALAPROPRO
12   SERHISLEUILEARGVALGLUGLYASNSER
13   HISALAGLNTYRVALGLUASPPROILETHR
14   GLYARGGLNSERVALLEUVALPROTYRGLU
15   PROPROGLNVALGLYTHRGLUPHETHRTHR
16   VALLEUTYRASNPHEMETCYSASNSERSER
17   CYSVALGLYGLYMETASNARGARGPROILE
18   LEUILEILEVALTHRLEUGLUTHRARGASP
19   GLYGLNVALLEUGLYARGARGCYSPHEGLU
20   ALAARGILECYSALACYSPROGLYARGASP
21   ARGLYSALAASPGLUASPSERILEARGLYS
22   GLNGLNVALSERASPSERTHRLYSASNGLY
23   ASPALAPHEARGGLNASNTHRHISGLYILE
24   GLNMETTHR

Samples:

sample_1: p63, [U-75% 2H; U-98% 13C; U-98% 15N], 0.7 mM

sample_2: p63, [U-98% 15N], 0.7 mM

Ex_cond_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCnot availablenot availableEx_cond_1
HNHAnot availablenot availableEx_cond_1
1H,15N HSQC-TOCSYnot availablenot availableEx_cond_1
1H,15N HSQC-NOESYnot availablenot availableEx_cond_1
1H,15N HSQC-NOESY-1H,15N HSQCnot availablenot availableEx_cond_1
HNCOnot availablenot availableEx_cond_1
tr-HNCAnot availablenot availableEx_cond_1
tr-HN(CA)COnot availablenot availableEx_cond_1
tr-HN(CO)CACBnot availablenot availableEx_cond_1
tr-HNCACBnot availablenot availableEx_cond_1

Software:

xwinnmr v3.5 -

AURELIA v2.8.4 -

PASTA - automatic assignment program

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAA32432 BAA32433 BAA32592 BAA32593 BAB20631
EMBL CAA76562 CAB88216 CAC37098 CAC37099 CAC37100
GB AAC24830 AAC43038 AAC62633 AAC62634 AAC62635
REF NP_001079107 NP_001108450 NP_001108451 NP_001108452 NP_001108453
SP O88898 Q9H3D4 Q9JJP6
TPG DAA33389
AlphaFold O88898 Q9H3D4 Q9JJP6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks