BMRB Entry 5603

Title:
Chemical shift assignment for human proguanylin
Deposition date:
2002-11-28
Original release date:
2003-08-01
Authors:
Lauber, Thomas; Neudecker, Philipp; Roesch, Paul; Marx, Ute
Citation:

Citation: Lauber, Thomas; Neudecker, Philipp; Rosch, Paul; Marx, Ute. "Solution Structure of Human Proguanylin: The Role of a Hormone Prosequence"  J. Biol. Chem. 278, 24118-24124 (2003).
PubMed: 12707255

Assembly members:

Assembly members:
proguanylin, polymer, 96 residues, 10491 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts635
13C chemical shifts268
15N chemical shifts95
coupling constants30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1proguanylin1

Entities:

Entity 1, proguanylin 96 residues - 10491 Da.

1   GLYPROVALTHRVALGLNASPGLYASNPHE
2   SERPHESERLEUGLUSERVALLYSLYSLEU
3   LYSASPLEUGLNGLUPROGLNGLUPROARG
4   VALGLYLYSLEUARGASNPHEALAPROILE
5   PROGLYGLUPROVALVALPROILELEUCYS
6   SERASNPROASNPHEPROGLUGLULEULYS
7   PROLEUCYSLYSGLUPROASNALAGLNGLU
8   ILELEUGLNARGLEUGLUGLUILEALAGLU
9   ASPPROGLYTHRCYSGLUILECYSALATYR
10   ALAALACYSTHRGLYCYS

Samples:

sample_1: proguanylin, [U-15N; U-13C], 0.8 – 1.1 mM; phosphate buffer 100 mM

ex-cond_1: pH: 6.0; temperature: 293 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableex-cond_1
1H-15N NOESY-HSQCsample_1not availableex-cond_1
1H-15N TOCSY-HSQCsample_1not availableex-cond_1
HNHAsample_1not availableex-cond_1
15N HMQC-NOESY-HSQCsample_1not availableex-cond_1
HNCOsample_1not availableex-cond_1
HNCAsample_1not availableex-cond_1
HNCACBsample_1not availableex-cond_1
CBCA(CO)NHsample_1not availableex-cond_1
HBHA(CBCACO)NHsample_1not availableex-cond_1
H(CCO)NHsample_1not availableex-cond_1
C(CO)NHsample_1not availableex-cond_1
13C-CTHSQCsample_1not availableex-cond_1
13C-NOESY-HSQCsample_1not availableex-cond_1

Software:

NMRview v5.0.4 -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
EMBL CAC22258
GB AAA35915 AAA58625 AAI40429 AAI56567 EAX07168
REF NP_291031 XP_003812931
SP Q02747
AlphaFold Q02747

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks