BMRB Entry 5385

Title:
Chemical shift assignments for the 8OG:G mismatched duplex
Deposition date:
2002-06-05
Original release date:
2003-02-20
Authors:
Thiviyanathan, Varatharasa; Somasunderam, Anoma; Hazra, Tapas; Mitra, Sankar; Gorenstein, David
Citation:

Citation: Thiviyanathan, Varatharasa; Somasunderam, Anoma; Hazra, Tapas; Mitra, Sankar; Gorenstein, David. "Solution Structure of a DNA Duplex containing 8-hydroxy-2'-deoxyguanosine Opposite Deoxyguanosine"  J. Mol. Biol. 325, 433-442 (2003).
PubMed: 12498794

Assembly members:

Assembly members:
DNA, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA: CGCGAATTGGCG

Data sets:
Data typeCount
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
18-OG 11
28-OG 21

Entities:

Entity 1, 8-OG 1 12 residues - Formula weight is not available

1   DCDGDCDGDADADTDTDGDG
2   DCDG

Samples:

sample_1: DNA1.8 – 2.2 mM

condition_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablecondition_1
DQF-COSYsample_1not availablecondition_1
2D-TOCSYsample_1not availablecondition_1
31P-1H HETCORsample_1not availablecondition_1
HEHAHAsample_1not availablecondition_1

Software:

AMBER v6.0 - Molecular Dynamics

NMR spectrometers:

  • Varian Unity-Plus 750 MHz
  • Varian Unity-Plus 600 MHz