BMRB Entry 5272

Title:
1H, 13C and 15N chemical shift assignments of Schistocerca gregaria chymotrypsin inhibitor
Deposition date:
2002-02-05
Original release date:
2011-08-10
Authors:
Gaspari, Zoltan; Patthy, Andras; Graf, Laszlo; Patthy, Andras; Szenthe, Borbala; Nagy, Attila
Citation:

Citation: Gaspari, Zoltan; Patthy, Andras; Graf, Laszlo; Patthy, Andras. "Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria "  Eur. J. Biochem. 269, 527-537 (2002).
PubMed: 11856311

Assembly members:

Assembly members:
Schistocerca gregaria chymotrypsin inhibitor, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: desert locust   Taxonomy ID: 7010   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistocerca Gregaria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: SGTMCI-pET17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Schistocerca gregaria chymotrypsin inhibitor: EVTCEPGTTFKDKCNTCRCG SDGKSAACTLKACPQ

Data sets:
Data typeCount
1H chemical shifts198
15N chemical shifts31
T1 relaxation values30
T2 relaxation values30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sgci1

Entities:

Entity 1, sgci 35 residues - Formula weight is not available

1   GLUVALTHRCYSGLUPROGLYTHRTHRPHE
2   LYSASPLYSCYSASNTHRCYSARGCYSGLY
3   SERASPGLYLYSSERALAALACYSTHRLEU
4   LYSALACYSPROGLN

Samples:

sample_1: Schistocerca gregaria chymotrypsin inhibitor 2.5 mM

sample_2: Schistocerca gregaria chymotrypsin inhibitor, [U-15N], 1.5 mM

ex-cond_1: pH: 3.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
3D TOCSY-HSQCnot availablenot availablenot available
3D NOESY-HSQCnot availablenot availablenot available
15N T1not availablenot availablenot available
15N T2not availablenot availablenot available
1H,15N NOEnot availablenot availablenot available

Software:

TRIAD (SYBYL) v6.6, Tripos, Inc. - peak assignment

XEASY v1.3.6, ETH Zurich - peak assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

SWISS-PROT O46162
EMBL CAA70818
PDB
BMRB 6881 6880

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks