BMRB Entry 5269

Title:
Assignment of the 1H, 13C, and 15N signals of Synechocystis sp. PCC 6803 methemoglobin
Deposition date:
2002-01-31
Original release date:
2002-08-23
Authors:
Falzone, Christopher; Lecomte, Juliette
Citation:

Citation: Falzone, Christopher; Lecomte, Juliette. "Letter to the Editor: Assignment of the 1H, 13C, and 15N signals of Synechocystis sp. PCC 6803 methemoglobin"  J. Biomol. NMR 23, 71-72 (2002).
PubMed: 12061721

Assembly members:

Assembly members:
cyanobacterial hemoglobin, polymer, 124 residues, 14353 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Synechocystis sp. PCC 6803   Taxonomy ID: 1148   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Synechocystis PCC 6803

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts494
15N chemical shifts130
1H chemical shifts807

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlbN1
2Heme2

Entities:

Entity 1, GlbN 124 residues - 14353 Da.

1   METSERTHRLEUTYRGLULYSLEUGLYGLY
2   THRTHRALAVALASPLEUALAVALASPLYS
3   PHETYRGLUARGVALLEUGLNASPASPARG
4   ILELYSHISPHEPHEALAASPVALASPMET
5   ALALYSGLNARGALAHISGLNLYSALAPHE
6   LEUTHRTYRALAPHEGLYGLYTHRASPLYS
7   TYRASPGLYARGTYRMETARGGLUALAHIS
8   LYSGLULEUVALGLUASNHISGLYLEUASN
9   GLYGLUHISPHEASPALAVALALAGLUASP
10   LEULEUALATHRLEULYSGLUMETGLYVAL
11   PROGLUASPLEUILEALAGLUVALALAALA
12   VALALAGLYALAPROALAHISLYSARGASP
13   VALLEUASNGLN

Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: cyanobacterial hemoglobin, [U-13C; U-15N], 1 – 1.5 mM; phosphate 20 mM; H2O 95%; D2O 5%

sample_2: cyanobacterial hemoglobin, [U-15N], 1 – 1.5 mM; phosphate 20 mM; H2O 95%; D2O 5%

sample_3: cyanobacterial hemoglobin1 – 2 mM; phosphate 20 mM; H2O 95%; D2O 5%

sample_4: cyanobacterial hemoglobin1 – 2 mM; phosphate 20 mM; D2O 100%

ex-cond_1: pH: 7.5 na; temperature: 298 K

ex-cond_2: pH*: 7.5 na; temperature: 298 K

ex-cond_3: pH: 6.9 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2QF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
1H-15N-NOESYnot availablenot availablenot available
1H-15N-TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
1H-13C HSQC-NOESYnot availablenot availablenot available
HCC(CO)NHnot availablenot availablenot available
1H-15N HMQCnot availablenot availablenot available
1H-13C HMQCnot availablenot availablenot available

Software:

NMRPipe - manual peak assignment

Felix 97.0 - cross-peak volume determination

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA17991
REF NP_441311
SWISS-PROT P73925

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks