BMRB Entry 5244

Title:
Backbone Assignment of Endonuclease V from Bacteriophage T4 with deuterium labeling
Deposition date:
2001-12-26
Original release date:
2002-08-22
Authors:
Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin
Citation:

Citation: Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin. "Letter to the Editor: Backbone Assignments for Endonuclease V from Bacteriophage T4 with deuterium labeling "  J. Biomol. NMR 22, 383-384 (2002).
PubMed: 12018491

Assembly members:

Assembly members:
endonuclease V, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: T4   Taxonomy ID: 10665   Superkingdom: Viruses   Kingdom: not available   Genus/species: T4-like phages Bacteriophage T4

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
1H chemical shifts253
13C chemical shifts395
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1endonuclease V1

Entities:

Entity 1, endonuclease V 138 residues - Formula weight is not available

1   METTHRARGILEASNLEUTHRLEUVALSER
2   GLULEUALAASPGLNHISLEUMETALAGLU
3   TYRARGGLULEUPROARGVALPHEGLYALA
4   VALARGLYSHISVALALAASNGLYLYSARG
5   VALARGASPPHELYSILESERPROTHRPHE
6   ILELEUGLYALAGLYHISVALTHRPHEPHE
7   TYRASPLYSLEUGLUPHELEUARGLYSARG
8   GLNILEGLULEUILEALAGLUCYSLEULYS
9   ARGGLYPHEASNILELYSASPTHRTHRVAL
10   GLNASPILESERASPILEPROGLNGLUPHE
11   ARGGLYASPTYRILEPROHISGLUALASER
12   ILEALAILESERGLNALAARGLEUASPGLU
13   LYSILEALAGLNARGPROTHRTRPTYRLYS
14   TYRTYRGLYLYSALAILETYRALA

Samples:

sample_1: endonuclease V, [U-15N]-Ala, Asp, Arg, Gly, Lys, Ser, Thr, Tyr, Val, 1.0 mM

sample_2: endonuclease V, [U-15N], 2.0 mM

sample_3: endonuclease V, [U-70% 2H ; U-15N], 1.5 mM

sample_4: endonuclease V, [U-90% 2H; U-13C; U-15N], 1.5 mM

Ex-cond: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-15N NOESYnot availablenot availablenot available
3D 1H-15N TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HN(CO)CACBnot availablenot availablenot available
3D HNCOnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRDraw - data processing

NMRView - peak assignments

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA28215
GB AAA72721 AAC42096 AAD42563 ABX11097 ACL78337
REF NP_049733 YP_001595242 YP_002922460 YP_003734258
SP P04418
AlphaFold P04418

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks