BMRB Entry 5160

Title:
Microcin from Escherichia coli
Deposition date:
2001-09-25
Original release date:
2001-10-17
Authors:
Blond, A.; Cheminant, M.; Segalas-Milazzo, I.; Peduzzi, J.; Barthelemy, M.; Goulard, C.; Salomon, R.; Moreno, F.; Farias, R.; Rebuffat, S.
Citation:

Citation: Blond, A.; Cheminant, M.; Segalas-Milazzo, I.; Peduzzi, J.; Barthelemy, M.; Goulard, C.; Salomon, R.; Moreno, F.; Farias, R.; Rebuffat, S.. "Solution structure of microcin J25, the single macrocyclic antimicrobial peptide from Escherichia coli"  Eur. J. Biochem. 268, 2124-2133 (2001).
PubMed: 11277936

Assembly members:

Assembly members:
microcin J25, polymer, 21 residues, 2107 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
microcin J25: VGIGTPISFYGGGAGHVPEY F

Data sets:
Data typeCount
13C chemical shifts79
1H chemical shifts133
coupling constants31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1microcin J251

Entities:

Entity 1, microcin J25 21 residues - 2107 Da.

1   VALGLYILEGLYTHRPROILESERPHETYR
2   GLYGLYGLYALAGLYHISVALPROGLUTYR
3   PHE

Samples:

sample_1: microcin J25 6 mM; CD3OH 100%

sample_cond_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1
1H-13C HSQCsample_1not availablesample_cond_1
1H13C HMBCsample_1not availablesample_cond_1

Software:

xwinnmr v2.5 - processing, spectrum acquisition

AURELIA v2.5.9 - NOE data quantification

X-PLOR v3.851 - simulated annealing, three-dimensional structure calculation

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker AVANCE 400 MHz