BMRB Entry 50353

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50353

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Title:
1H, 13C, 15N chemical shift assignment of NTD MaSp2 from Nephila clavipes
Deposition date:
2020-06-23
Original release date:
2020-08-11
Authors:
Oktaviani, Nur Alia; Malay, Ali D; Matsugami, Akimasa; Hayashi, Fumiaki; Numata, Keiji
Citation:

Citation: Oktaviani, Nur Alia; Malay, Ali D; Matsugami, Akimasa; Hayashi, Fumiaki; Numata, Keiji. "Nearly complete 1H, 13C and 15N chemical shift assignment of monomeric form of N-terminal domain of Nephila clavipes major ampullate spidroin 2"  Biomol. NMR Assignments 14, 335-338 (2020).
PubMed: 32767002

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nephila clavipes   Taxonomy ID: 2585209   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nephila clavipes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMAQARSPWSDTATADAF IQNFLAAVSGSGAFTSDQLD DMSTIGDTIMSAMDKMARSN KSSQHKLQALNMAFASSMAE IAAVEQGGMSMAVKTNAIVD GLNSAFYMTTGAANPQFVNE MRSLISMISAASANEVSY

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts143
1H chemical shifts881

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain of Major Ampullate Spidroin 21

Entities:

Entity 1, N-terminal domain of Major Ampullate Spidroin 2 138 residues - Formula weight is not available

1   GLYSERHISMETALAGLNALAARGSERPRO
2   TRPSERASPTHRALATHRALAASPALAPHE
3   ILEGLNASNPHELEUALAALAVALSERGLY
4   SERGLYALAPHETHRSERASPGLNLEUASP
5   ASPMETSERTHRILEGLYASPTHRILEMET
6   SERALAMETASPLYSMETALAARGSERASN
7   LYSSERSERGLNHISLYSLEUGLNALALEU
8   ASNMETALAPHEALASERSERMETALAGLU
9   ILEALAALAVALGLUGLNGLYGLYMETSER
10   METALAVALLYSTHRASNALAILEVALASP
11   GLYLEUASNSERALAPHETYRMETTHRTHR
12   GLYALAALAASNPROGLNPHEVALASNGLU
13   METARGSERLEUILESERMETILESERALA
14   ALASERALAASNGLUVALSERTYR

Samples:

sample_1: N-terminal domain of Major Ampullate Spidroin 2, [U-100% 13C; U-100% 15N], 0.75 mM; D2O, [U-100% 2H], 10%; DSS 0.1 mM; potassium phosphate pH 7 10 mM; sodium chloride 300 mM

sample_conditions_1: ionic strength: 0.301 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOCAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D (H)C(CO)NHsample_1isotropicsample_conditions_1
3D H(C)CH TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH TOCSYsample_1isotropicsample_conditions_1
2D H2(C)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESY HSQCsample_1isotropicsample_conditions_1
2D CG(CB)HBsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker ECA 700 MHz
  • Bruker ECA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks