Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50238
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Citation: Olivieri, Cristina; Wang, Yingjie; Li, Geoffrey; Veliparambil Subrahmanian, Manu; Kim, Jonggul; Stultz, Benjamin; Neibergall, Matthew; Porcelli, Fernando; Muretta, Joseph; Thomas, David; Gao, Jiali; Blumenthal, Donald; Taylor, Susan; Veglia, Gianluigi. "Multi-state recognition pathway of the intrinsically disordered protein kinase inhibitor by protein kinase A" eLIFE 9, e55607-e55607 (2020).
PubMed: 32338601
Assembly members:
entity_1, polymer, 75 residues, 7857.33 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7-7
Entity Sequences (FASTA):
entity_1: TDVETTYADFIASGRTGRRN
AIHDILVSSASGNSNELALK
LAGLDINKTEGEEDAQRSST
EQSGEAQGEAAKSES
Data type | Count |
13C chemical shifts | 208 |
15N chemical shifts | 74 |
1H chemical shifts | 425 |
T1 relaxation values | 73 |
T2 relaxation values | 73 |
heteronuclear NOE values | 70 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PKIA | 1 |
Entity 1, PKIA 75 residues - 7857.33 Da.
1 | THR | ASP | VAL | GLU | THR | THR | TYR | ALA | ASP | PHE | ||||
2 | ILE | ALA | SER | GLY | ARG | THR | GLY | ARG | ARG | ASN | ||||
3 | ALA | ILE | HIS | ASP | ILE | LEU | VAL | SER | SER | ALA | ||||
4 | SER | GLY | ASN | SER | ASN | GLU | LEU | ALA | LEU | LYS | ||||
5 | LEU | ALA | GLY | LEU | ASP | ILE | ASN | LYS | THR | GLU | ||||
6 | GLY | GLU | GLU | ASP | ALA | GLN | ARG | SER | SER | THR | ||||
7 | GLU | GLN | SER | GLY | GLU | ALA | GLN | GLY | GLU | ALA | ||||
8 | ALA | LYS | SER | GLU | SER |
sample_1: cAMP-dependent protein kinase A Inhibitor alpha (PKIA), [U-13C; U-15N], 0.65 mM; potassium phosphate 20 mM; potassium chloride 90 mM; magnesium chloride 10 mM; DTT 10 mM; sodium azide 1 mM
sample_2: cAMP-dependent protein kinase A Inhibitor alpha (PKIA), [U-15N; U-2H], 0.45 mM; potassium phosphate 20 mM; potassium chloride 90 mM; magnesium chloride 10 mM; DTT 10 mM; sodium azide 1 mM
sample_3: cAMP-dependent protein kinase A Inhibitor alpha (PKIA), [U-15N], 0.3 mM; potassium phosphate 20 mM; potassium chloride 90 mM; magnesium chloride 10 mM; DTT 10 mM; sodium azide 1 mM
sample_conditions_1: ionic strength: 0.24 M; pH: 6.5; pressure: 1 atm; temperature: 300 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY-HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CC(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_2 | isotropic | sample_conditions_1 |
1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
RDC | sample_3 | anisotropic | sample_conditions_1 |
RDC | sample_3 | anisotropic | sample_conditions_1 |
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