BMRB Entry 50123

Title:
Backbone 13C, and 15N Chemical Shift Assignments for KirBac1.1
Deposition date:
2019-12-11
Original release date:
2019-12-19
Authors:
Amani, Reza
Citation:

Citation: Amani, Reza; Borcik, Collin; Khan, Nazmul; Versteeg, Derek; Yekefallah, Maryam; Do, Hoa; Coats, Heather; Wylie, Benjamin. "Conformational changes upon gating of KirBac1.1 into an open-activated state revealed by solid-state NMR and functional assays"  Proc. Natl. Acad. Sci. U.S.A. 117, 2938-2947 (2020).
PubMed: 31980523

Assembly members:

Assembly members:
entity_1, polymer, 345 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Burkholderia Pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia Pseudomallei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE60

Data sets:
Data typeCount
13C chemical shifts1311
15N chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kirbac1.1, chain 11
2Kirbac1.1, chain 21
3Kirbac1.1, chain 31
4Kirbac1.1, chain 41

Entities:

Entity 1, Kirbac1.1, chain 1 345 residues - Formula weight is not available

1   METASNVALASPPROPHESERPROHISSER
2   SERASPSERPHEALAGLNALAALASERPRO
3   ALAARGLYSPROPROARGGLYGLYARGARG
4   ILETRPSERGLYTHRARGGLUVALILEALA
5   TYRGLYMETPROALASERVALTRPARGASP
6   LEUTYRTYRTRPALALEULYSVALSERTRP
7   PROVALPHEPHEALASERLEUALAALALEU
8   PHEVALVALASNASNTHRLEUPHEALALEU
9   LEUTYRGLNLEUGLYASPALAPROILEALA
10   ASNGLNSERPROPROGLYPHEVALGLYALA
11   PHEPHEPHESERVALGLUTHRLEUALATHR
12   VALGLYTYRGLYASPMETHISPROGLNTHR
13   VALTYRALAHISALAILEALATHRLEUGLU
14   CYSPHEVALGLYMETSERGLYILEALALEU
15   SERTHRGLYLEUVALPHEALAARGPHEALA
16   ARGPROARGALALYSILEMETPHEALAARG
17   HISALAILEVALARGPROPHEASNGLYARG
18   METTHRLEUMETVALARGALAALAASNALA
19   ARGGLNASNVALILEALAGLUALAARGALA
20   LYSMETARGLEUMETARGARGGLUHISSER
21   SERGLUGLYTYRSERLEUMETLYSILEHIS
22   ASPLEULYSLEUVALARGASNGLUHISPRO
23   ILEPHELEULEUGLYTRPASNMETMETHIS
24   VALILEASPGLUSERSERPROLEUPHEGLY
25   GLUTHRPROGLUSERLEUALAGLUGLYARG
26   ALAMETLEULEUVALMETILEGLUGLYSER
27   ASPGLUTHRTHRALAGLNVALMETGLNALA
28   ARGHISALATRPGLUHISASPASPILEARG
29   TRPHISHISARGTYRVALASPLEUMETSER
30   ASPVALASPGLYMETTHRHISILEASPTYR
31   THRARGPHEASNASPTHRGLUPROVALGLU
32   PROPROGLYALAALAPROASPALAGLNALA
33   PHEALAALALYSPROGLYGLUGLYASPALA
34   ARGPROVALPROARGGLYSERARGSERHIS
35   HISHISHISHISHIS

Samples:

sample_1: entity_1, [U-13C; U-15N], 25%; H2O 55.55 M; POPC 66%; POPG 33%; KCl 50 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CANcoCAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • JEOL ASC 600 MHz