BMRB Entry 50097

Title:
1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications
Deposition date:
2019-11-28
Original release date:
2020-09-19
Authors:
Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre
Citation:

Citation: Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre. "1H, 15N chemical shift assignments of the imino groups of yeast tRNA Phe: influence of the post-transcriptional modifications"  Biomol. NMR Assignments 14, 169-174 (2020).
PubMed: 32239363

Assembly members:

Assembly members:
matured yeast tRNAPhe, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts28
1H chemical shifts28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tRNAPhe1

Entities:

Entity 1, tRNAPhe 76 residues - Formula weight is not available

1   GCGGAUUUAM2G
2   CUCAGDDGGG
3   AGAGCM22GCCAG
4   ACMUGMAAYWAPSUM5C
5   UGGAGM7GUCM5CU
6   GUGM5UPSUCGM1AUC
7   CACAGAAUUC
8   GCACCA

Samples:

sample_1: entity_1 0.8 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N BEST-TROSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

tRNADB tdbR00000083