BMRB Entry 50025

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB)
Deposition date:
2019-09-25
Original release date:
2020-03-27
Authors:
Bartkevihi, Leonardo; Almeida, Fabio; Ano Bom, Cristiane
Citation:

Citation: Bartkevihi, Leonardo; Martins, Bruna; de Oliveira, Danielle Maria Perpetua; Pires, Jose Ricardo; Anobom, Cristiane Dinis; Almeida, Fabio. "Backbone assignment of ribose-5-phosphate isomerase of Mycobacterium tuberculosis (MtRpiB)"  Biomol. NMR Assignments 14, 119-122 (2020).
PubMed: 32030620

Assembly members:

Assembly members:
entity_1, polymer, 170 residues, 18312.6696 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCRT7/NT-TOPO

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Molecule 1, 11
2Molecule 1, 21

Entities:

Entity 1, Molecule 1, 1 170 residues - 18312.6696 Da.

1   METALAHISHISHISHISHISHISMETSER
2   GLYMETARGVALTYRLEUGLYALAASPHIS
3   ALAGLYTYRGLULEULYSGLNARGILEILE
4   GLUHISLEULYSGLNTHRGLYHISGLUPRO
5   ILEASPCYSGLYALALEUARGTYRASPALA
6   ASPASPASPTYRPROALAPHECYSILEALA
7   ALAALATHRARGTHRVALALAASPPROGLY
8   SERLEUGLYILEVALLEUGLYGLYSERGLY
9   ASNGLYGLUGLNILEALAALAASNLYSVAL
10   PROGLYALAARGCYSALALEUALATRPSER
11   VALGLNTHRALAALALEUALAARGGLUHIS
12   ASNASNALAGLNLEUILEGLYILEGLYGLY
13   ARGMETHISTHRVALALAGLUALALEUALA
14   ILEVALASPALAPHEVALTHRTHRPROTRP
15   SERLYSALAGLNARGHISGLNARGARGILE
16   ASPILELEUALAGLUTYRGLUARGTHRHIS
17   GLUALAPROPROVALPROGLYALAPROALA

Samples:

sample_1: TRIS 20 mM; sodium chloride 100 mM; DTT 5 mM; sodium azide 5 mM; EDTA 5 mM; PMSF 2 mM; MtRpiB, [U-13C; U-15N; U-2H], 700 uM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4, CCPN - chemical shift assignments

DANGLE v1.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks